Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
Address correspondence to: Terry B. Strom, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, E/CLS 608, Harvard Medical School, Boston, Massachusetts 02215, USA. Phone: 617.735.2880; Fax: 617.667.0923; E-mail: firstname.lastname@example.org.
First published May 15, 2013 - More info
The migration of effector or memory T cells to the graft is a critical event in the rejection of transplanted organs. The prevailing view is that the key steps involved in T cell migration — integrin-mediated firm adhesion followed by transendothelial migration — are dependent on the activation of Gαi-coupled chemokine receptors on T cells. In contrast to this view, we demonstrated in vivo that cognate antigen was necessary for the firm adhesion and transendothelial migration of CD8+ effector T cells specific to graft antigens and that both steps occurred independent of Gαi signaling. Presentation of cognate antigen by either graft endothelial cells or bone marrow–derived APCs that extend into the capillary lumen was sufficient for T cell migration. The adhesion and transmigration of antigen-nonspecific (bystander) effector T cells, on the other hand, remained dependent on Gαi, but required the presence of antigen-specific effector T cells. These findings underscore the primary role of cognate antigen presented by either endothelial cells or bone marrow–derived APCs in the migration of T cells across endothelial barriers and have important implications for the prevention and treatment of graft rejection.
Jeffrey M. Walch, Qiang Zeng, Qi Li, Martin H. Oberbarnscheidt, Rosemary A. Hoffman, Amanda L. Williams, David M. Rothstein, Warren D. Shlomchik, Jiyun V. Kim, Geoffrey Camirand, Fadi G. Lakkis
During transplant rejection, migrating T cells infiltrate the grafted organ, but the signals that direct this migration are incompletely understood. In this issue of the JCI, Walch et al. debunk two classical paradigms concerning transplant rejection, with important consequences for the design of antirejection therapeutics.
Transplant rejection begins with the migration and influx of recipient T cells into the transplant. Many, but not all, of these T cells bear TCRs specific for donor alloantigen. If migration to and infiltration of the transplant by tissue-damaging anti-donor T cells can be neutralized, even temporarily, the recipient anti-donor allograft response would be significantly weakened and more amenable to low-dose or tolerizing therapies.
The current dogma explaining the initial phases of the allograft response is based on the leukocyte migration paradigm (1). Chemokines, a family of small transmembrane proteins that attract migratory T cells bearing specific receptors for them, are believed to draw T cells to the transplant. Then, the interaction of T cell surface integrins with molecules present on donor endothelial cells retain T cells and enable their transmigration into the transplant. When chemokines interact with chemokine receptors, chemokine receptor–associated Gαi proteins are activated, and a pertussis toxin–sensitive signaling cascade is initiated. The downstream Gαi signaling pathway induces conformational changes in the integrins expressed on chemokine-activated T cells (2). These changes enhance the affinity of integrins such as VLA-4 for surrounding tissues, thought to be endothelial venules, of the transplant (Figure 1).
Integrin avidity determines T cell infiltration of transplants. (A) Low-affinity integrins in resting T cells are converted to a high-affinity state by exposure to chemokines, also a stimulus for migration of non–donor-reactive T cells to the transplant. (B) Integrin affinity may also be altered by antigen recognition, which initiates infiltration of the transplant by T cells bearing TCRs for transplant antigens. Figure adapted with permission from Cellular and Molecular Immunology (14).
Chemokine production is markedly amplified within inflamed tissues, and transplant procedures, especially those involving deceased donors, inevitably lead to ischemia-reperfusion injury, resulting in inflammation of the endothelium and chemokine expression (3, 4). Thus, it has been widely believed that chemokines, which regulate T cell traffic in lymphoid tissue, also play a pivotal role in transplant rejection.
Against this theoretical backdrop, and influenced by the surprisingly weak therapeutic effect of antibodies directed against chemokine receptors upon transplant survival (5–7), Walch et al. tested the authenticity of the classical leukocyte migration paradigm in mouse cardiac and renal transplant models (8). In short, they confirmed an important role for the integrin VLA-4, but found that the paradigm did not hold true for the role of chemokines in directing the massive influx of recipient anti-donor T cells in the allograft response.
To investigate the role of chemokine receptors in the allograft response, Walch et al. used passive T cell transfer models in which recipient strain T cells are injected into syngeneic lymphopenic hosts. They found that infiltration into allografts by pertussis toxin–treated memory or activated effector CD8+ T cells previously stimulated with donor cells was unimpaired, whereas migration to lymph nodes was affected (8). Hence, migration of memory or activated effector CD8+ T cells to the allograft is not dependent upon activation of chemokine receptors. The same conclusions were reached in experiments analyzing rejection of newly engrafted organs, when inflammation caused by ischemia-reperfusion injury is present, and in hosts in which transfer of lymphocytes was delayed to 50 days after transplantation, after dissipation of ischemia-reperfusion–related inflammation. In these experiments, rejection mediated by pertussis toxin–treated donor-reactive memory T cells was only slightly delayed compared with controls (8).
That chemokine-stimulated migration of donor-reactive CD8+ activated effector or memory T cells to host lymphoid tissue is not a necessary prelude to infiltration and rejection of the transplant was corroborated by experiments in transplant recipients that lack secondary lymphoid tissues (e.g., lymph node and spleen). Interestingly, purified and passively transferred naive T cells or even memory-type T cells that do not recognize donor antigens did not infiltrate the transplant unless these non–donor-reactive T cells were cotransferred with donor-reactive T cells. Thus, in stark contrast to the previously accepted dogma, infiltration of donor-reactive CD8+ T cells into the transplant and rejection are not dependent upon chemokines.
If chemokines emanating from an inflamed endothelium are not involved in drawing donor-reactive T cells into the transplant, what is responsible for this crucially important step in the allograft response? Walch et al. confirmed an important role for integrins in enabling infiltration of the allograft, but their results made clear the chemokine receptor Gαi signaling pathway is not involved. Knowing that activation of the TCR complex, like activation of chemokine receptors, induces functionally critical conformational change in integrins, the authors studied the role of donor antigen in the influx of CD8+ donor-reactive and non–donor-reactive T cells. Again, a passive lymphocyte transfer system was used, but in this series of experiments, CD8+ T cells were harvested from mice bearing transgenic TCRs that do or do not recognize donor antigen. Both sets of T cells were able to infiltrate transplants; however, T cells that do not express receptors for donor antigen did not infiltrate transplants unless accompanied by TCR donor-reactive T cells. These T cell populations also differed in their requirements for chemokine receptor or TCR activation in infiltrating the transplant (Figure 1). Inactivation of the chemokine receptor–linked Gαi protein signaling pathway with pertussis toxin prevented migration of CD8+ T cells bearing the non–donor-reactive TCR complex, but not T cells bearing the donor-reactive TCR complex (8). Similar conclusions have emerged in an analysis of T cell migration in an autoimmune mouse model of type 1 diabetes (9, 10).
Walch et al. performed two-photon intravital imaging to further analyze infiltration by TCR transgenic CD8+ T cells. Donor antigen–initiated responses, but not chemokine receptor–initiated responses, were shown to be responsible for the migration and adhesion of CD8+ donor-reactive TCR T cells to the capillary lumina, which in turn led to transmigration of donor-reactive effector T cells into the transplant. Previously, donor endothelial cells were believed to be solely responsible for presentation of antigen to T cells migrating into the vascular lumen (11). However, Walch et al. found that bone marrow–derived APCs such as dendritic cells were more effective in retaining T cells within the graft than were endothelial cells. Infiltration of non–donor-reactive T cells into the transplant was enabled by cotransfer with donor-reactive T cells (8).
Even perfect matching of major histocompatibility locus antigens between donor and recipient does not obviate the need for immunosuppressive therapy, because mismatches for the myriad of minor histocompatibility antigens can also lead to rejection. As blockade of chemokine/chemokine receptor interactions will not block the initial stages of rejection, an alternative approach might be to treat with antibodies directed at the TCR complex.
Indeed, treatment with antibodies directed at a nonpolymorphic component of the TCR complex provides a well-appreciated therapeutic option to block the initial stages of the allograft response. Upon TCR activation, CD3 proteins polymerize with T cell antigen receptor protein (reviewed in ref. 12). Stimulation of the TCR complex by anti-CD3 leads to polymerization with the TCR, transiently activating T cells. Subsequently, T cell activation leads to an outpouring of proinflammatory cytokines, before the TCR complex is lost from the cell surface and immunosuppressive and even immune tolerance–promoting effects are obtained. Attempts to obviate these side effects by structural changes in the antibody have proven partially, but not totally, successful (12, 13). Further improvements in antibody design or adaptation of multiagent strategies that enable successful treatment with lower and safer doses of anti-CD3 are required. The work of Walch et al. (8) serves to refocus attention on the initiation of the allograft response from the chemokine/chemokine receptor pathway toward activation of the TCR complex. While paradigms may be dashed, this work elucidates the molecular basis of the initial stages of the allograft response. This newly obtained and more complete understanding of this process will have substantial implications for transplant medicine.
T.B. Strom is funded by grants awarded by the NIH (NIAID and NIDDK) and the Juvenile Diabetes Research Foundation.
Conflict of interest: The author has declared that no conflict of interest exists.
Reference information: J Clin Invest. 2013;123(6):2360–2362. doi:10.1172/JCI69385.
See the related article at Cognate antigen directs CD8+ T cell migration to vascularized transplants.