Ablation of the PTHrP gene or the PTH/PTHrP receptor gene leads to distinct abnormalities in bone development
Beate Lanske, Michael Amling, Lynn Neff, Jennifer Guiducci, Roland Baron, Henry M. Kronenberg
Beate Lanske, Michael Amling, Lynn Neff, Jennifer Guiducci, Roland Baron, Henry M. Kronenberg
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Article

Ablation of the PTHrP gene or the PTH/PTHrP receptor gene leads to distinct abnormalities in bone development

Abstract

Parathyroid hormone (PTH) and parathyroid hormone–related peptide (PTHrP) bind to and activate the same PTH/PTHrP receptor. Deletion of either the PTHrP gene or the PTH/PTHrP receptor gene leads to acceleration of differentiation of growth plate chondrocytes. To explore further the functional relationships of PTHrP and the PTH/PTHrP receptor, bones of knockout mice were analyzed early in development, and the phenotypes of double-knockout mice were characterized.

Authors

Beate Lanske, Michael Amling, Lynn Neff, Jennifer Guiducci, Roland Baron, Henry M. Kronenberg

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Chondrocyte differentiation at E16.5. Hematoxylin/eosin staining (a–c) a...
Chondrocyte differentiation at E16.5. Hematoxylin/eosin staining (a–c) and type X collagen mRNA in situ hybridization (d–f) of wild-type, PTH/PTHrP-R–/–, and PTHrP–/– phalanges at E16.5. In contrast to PTHrP–/– bones, which exhibit a spatial and temporal progression in chondrocyte differentiation (c and f), PTH/PTHrP-R–/– bones show a delay in chondrocyte differentiation, as shown by the decrease in type X collagen expression (b and e), when compared with wild-type bones (a and d). However, the spatial distribution of the abnormally differentiated hypertrophic cells is identical (b, c and e, f).