Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
  • Current Issue
  • Past Issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • 100th Anniversary of Insulin's Discovery (Jan 2021)
    • Hypoxia-inducible factors in disease pathophysiology and therapeutics (Oct 2020)
    • Latency in Infectious Disease (Jul 2020)
    • Immunotherapy in Hematological Cancers (Apr 2020)
    • Big Data's Future in Medicine (Feb 2020)
    • Mechanisms Underlying the Metabolic Syndrome (Oct 2019)
    • Reparative Immunology (Jul 2019)
    • View all review series ...
  • Viewpoint
  • Collections
    • Recently published
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • Recently published
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
A novel murine infection model for Shiga toxin–producing Escherichia coli
Emily M. Mallick, … , John M. Leong, David B. Schauer
Emily M. Mallick, … , John M. Leong, David B. Schauer
Published October 8, 2012
Citation Information: J Clin Invest. 2012;122(11):4012-4024. https://doi.org/10.1172/JCI62746.
View: Text | PDF
Technical Advance Infectious disease

A novel murine infection model for Shiga toxin–producing Escherichia coli

  • Text
  • PDF
Abstract

Enterohemorrhagic E. coli (EHEC) is an important subset of Shiga toxin–producing (Stx-producing) E. coli (STEC), pathogens that have been implicated in outbreaks of food-borne illness and can cause intestinal and systemic disease, including severe renal damage. Upon attachment to intestinal epithelium, EHEC generates “attaching and effacing” (AE) lesions characterized by intimate attachment and actin rearrangement upon host cell binding. Stx produced in the gut transverses the intestinal epithelium, causing vascular damage that leads to systemic disease. Models of EHEC infection in conventional mice do not manifest key features of disease, such as AE lesions, intestinal damage, and systemic illness. In order to develop an infection model that better reflects the pathogenesis of this subset of STEC, we constructed an Stx-producing strain of Citrobacter rodentium, a murine AE pathogen that otherwise lacks Stx. Mice infected with Stx-producing C. rodentium developed AE lesions on the intestinal epithelium and Stx-dependent intestinal inflammatory damage. Further, the mice experienced lethal infection characterized by histopathological and functional kidney damage. The development of a murine model that encompasses AE lesion formation and Stx-mediated tissue damage will provide a new platform upon which to identify EHEC alterations of host epithelium that contribute to systemic disease.

Authors

Emily M. Mallick, Megan E. McBee, Vijay K. Vanguri, Angela R. Melton-Celsa, Katherine Schlieper, Brad J. Karalius, Alison D. O’Brien, Joan R. Butterton, John M. Leong, David B. Schauer

×

Figure 1

C. rodentium (λstx2dact) produces high levels of Shiga toxin upon prophage induction at levels comparable to EHEC isolates.

Options: View larger image (or click on image) Download as PowerPoint

C. rodentium (λstx2dact) produces high levels of Shiga toxin upon proph...
Stx2 in supernatants (S) or pellets (P) of untreated or mitomycin C–treated cultures of the indicated strains was measured by capture ELISA, and results are expressed relative to bacterial number (see Methods). ND, not detected. Cr, C. rodentium. Data shown are the averages ± SEM of quadruplicate samples. Data show results of 1 experiment representative of 3 independent experiments.
Follow JCI:
Copyright © 2021 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts