NOD2-mediated dysbiosis predisposes mice to transmissible colitis and colorectal cancer
Aurélie Couturier-Maillard, Thomas Secher, Ateequr Rehman, Sylvain Normand, Adèle De Arcangelis, Robert Haesler, Ludovic Huot, Teddy Grandjean, Aude Bressenot, Anne Delanoye-Crespin, Olivier Gaillot, Stefan Schreiber, Yves Lemoine, Bernhard Ryffel, David Hot, Gabriel Nùñez, Grace Chen, Philip Rosenstiel, Mathias Chamaillard
Aurélie Couturier-Maillard, Thomas Secher, Ateequr Rehman, Sylvain Normand, Adèle De Arcangelis, Robert Haesler, Ludovic Huot, Teddy Grandjean, Aude Bressenot, Anne Delanoye-Crespin, Olivier Gaillot, Stefan Schreiber, Yves Lemoine, Bernhard Ryffel, David Hot, Gabriel Nùñez, Grace Chen, Philip Rosenstiel, Mathias Chamaillard
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Research Article Oncology

NOD2-mediated dysbiosis predisposes mice to transmissible colitis and colorectal cancer

Abstract

Instability in the composition of gut bacterial communities (dysbiosis) has been linked to common human intestinal disorders, such as Crohn’s disease and colorectal cancer. Here, we show that dysbiosis caused by Nod2 deficiency gives rise to a reversible, communicable risk of colitis and colitis-associated carcinogenesis in mice. Loss of either Nod2 or RIP2 resulted in a proinflammatory microenvironment that enhanced epithelial dysplasia following chemically induced injury. The condition could be improved by treatment with antibiotics or an anti–interleukin-6 receptor–neutralizing antibody. Genotype-dependent disease risk was communicable via maternally transmitted microbiota in both Nod2-deficient and WT hosts. Furthermore, reciprocal microbiota transplantation reduced disease risk in Nod2-deficient mice and led to long-term changes in intestinal microbial communities. Conversely, disease risk was enhanced in WT hosts that were recolonized with dysbiotic fecal microbiota from Nod2-deficient mice. Thus, we demonstrated that licensing of dysbiotic microbiota is a critical component of disease risk. Our results demonstrate that NOD2 has an unexpected role in shaping a protective assembly of gut bacterial communities and suggest that manipulation of dysbiosis is a potential therapeutic approach in the treatment of human intestinal disorders.

Authors

Aurélie Couturier-Maillard, Thomas Secher, Ateequr Rehman, Sylvain Normand, Adèle De Arcangelis, Robert Haesler, Ludovic Huot, Teddy Grandjean, Aude Bressenot, Anne Delanoye-Crespin, Olivier Gaillot, Stefan Schreiber, Yves Lemoine, Bernhard Ryffel, David Hot, Gabriel Nùñez, Grace Chen, Philip Rosenstiel, Mathias Chamaillard

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Figure 5

Broad-spectrum antibiotic treatment improves intestinal inflammation and tumorigenesis in Nod2-deficient mice.

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Broad-spectrum antibiotic treatment improves intestinal inflammation and...
Five days after AOM administration (at 12 mg/kg), Nod2+/+ (n = 4), Nod2–/– (n = 3), antibiotic-treated (Atb-treated) WT (n = 4), and Atb-treated Nod2–/– (n = 3) mice were subjected to 2% DSS for 7 days followed by 9-day access to regular drinking water. (A) Changes in body weight were monitored daily. (B) Colonic-associated Bacteroides load was determined by specific q-PCR analysis at day 16. (C–E) Ly6G, IA/IE, CD11c, and CD11b staining of the lamina propria cell population in Nod2–/– mice treated or not with antibiotics as indicated was quantified by FACS analysis. (F) Colon weight/length ratio quantification on Nod2+/+ (n = 10), Nod2–/– (n = 9), antibiotic-treated Nod2+/+ (n = 10) and antibiotic-treated Nod2–/– (n = 10) mice was performed at day 58. *P < 0.05; **P < 0.01; ***P < 0.001.