(A) Vm oscillatory voltage commands generated from voltage recordings to construct average cycles (see Methods) that represent control and Ang II oscillatory activity. 4 (control; 2.14 s) or 8 (Ang II; 1.24 s) Vm cycles were delivered in 10 s (top traces). Mean Ca²⁺ currents evoked sequentially by control and Ang II voltage commands (n = 16) are shown below. Also displayed are magnified representations of the mean current elicited during first and last cycles. (B) Cycle comparison of averaged peak Ca²⁺ currents and averaged Ca²⁺ current areas. Peak current and current area per cycle per cell (n = 16) were calculated and averaged. Average Ca²⁺ current area summed across cycle number (i.e., total current) evoked by Ang II command was 240% that of control command. (C) Ni²⁺-sensitive Cav3.2 Ca²⁺ current. Mean Ca²⁺ currents recorded in the absence or presence of 100 μM Ni²⁺ evoked by the first or last Vm cycle. The blue difference current defines the Ni²⁺-sensitive Cav3.2 current, which, because of incomplete block by Ni²⁺, was an underestimate. (D) Average peak Ca²⁺ current and average Ca²⁺ current area compared between first and last cycle, showing persistent Ni²⁺ block (n = 6). Average Ni²⁺-sensitive component (Cav3.2) of Ca²⁺ current area summed across cycle number (i.e., total Ni²⁺-sensitive current) evoked by Ang II command was 220% that of control. (E) Mean Ni²⁺-sensitive current averaged during interspike intervals elicited by Ang II command (n = 6). ⁺P < 0.05 vs. first cycle; *P < 0.05 vs. control. Bars represent mean ± SEM.