(A) VBIM strategy. (B) HME1 cells were infected with retroviruses encoding GFP^loxP and RAS^loxP and analyzed for AIG. Cells were recovered from agar and infected with a retrovirus encoding CRE recombinase (CRE) or a control retrovirus (Vec) and analyzed for AIG. (C) The screen was conducted using 10,000 cells per well and 6 wells for each of the 3 VBIM viruses. The initial 10,000 cells were expanded to 200,000 cells and analyzed for AIG. The 5 libraries with more than twice as many colonies as the GFP control are denoted by asterisks. Cells expressing GFP (–) or RAS (+) alone served as the negative and positive controls, respectively. (D) 5 pools were recovered from agar, infected with a retrovirus encoding CRE or control vector, and analyzed for AIG. (E) VBIM integration site in FAM83B. The number of nucleotides comprising each of the 5 exons of FAM83B is shown in blue. The number of nucleotides comprising each intron is shown in green. The number of amino acids encoded by each exon is shown in red. The yellow arrow denotes the VBIM insertion site within intron 2. (F) RT-PCR was performed on RNA from SD3-5 cells using a primer targeting the 5′ region of the VBIM-driven mRNA and a 3′ FAM83B-specific primer. (G) Northern analysis of FAM83B expression in parental HME1 cells and SD3-5 cells infected with a retrovirus encoding CRE or control vector. Lanes in F and G were run on the same gel but were noncontiguous (white lines).