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Amendment history:
  • Correction (April 1998)

Research Article Free access | 10.1172/JCI483

NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.

Y Iimuro, T Nishiura, C Hellerbrand, K E Behrns, R Schoonhoven, J W Grisham, and D A Brenner

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

Find articles by Iimuro, Y. in: PubMed | Google Scholar

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

Find articles by Hellerbrand, C. in: PubMed | Google Scholar

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

Find articles by Behrns, K. in: PubMed | Google Scholar

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

Find articles by Schoonhoven, R. in: PubMed | Google Scholar

Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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Published February 15, 1998 - More info

Published in Volume 101, Issue 4 on February 15, 1998
J Clin Invest. 1998;101(4):802–811. https://doi.org/10.1172/JCI483.
© 1998 The American Society for Clinical Investigation
Published February 15, 1998 - Version history
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Abstract

Although NFkappaB binding activity is induced during liver regeneration after partial hepatectomy, the physiological consequence of this induction is unknown. We have assessed the role of NFkappaB during liver regeneration by delivering to the liver a superrepressor of NFkappaB activity using an adenoviral vector expressing a mutated form of IkappaBalpha. This adenovirus (Ad5IkappaB) was almost exclusively expressed in the liver and inhibited NFkappaB DNA binding activity and transcriptional activity in cultured cells as well as in the liver in vivo. After partial hepatectomy, infection with Ad5IkappaB, but not a control adenovirus (Ad5LacZ), resulted in the induction of massive apoptosis and hepatocytes as demonstrated by histological staining and TUNEL analysis. In addition, infection with Ad5IkappaB but not Ad5LacZ decreased the mitotic index after partial hepatectomy. These two phenomena, increased apoptosis and failure to progress through the cell cycle, were associated with liver dysfunction in animals infected with the Ad5IkappaB but not Ad5LacZ, as demonstrated by elevated serum bilirubin and ammonia levels. Thus, the induction of NFkappaB during liver regeneration after partial hepatectomy appears to be a required event to prevent apoptosis and to allow for normal cell cycle progression.

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