Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York, USA.
Address correspondence to: Rachel. L. Miller, Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, PH8E, 630 W. 168th Street, New York, New York 10032, USA. Phone: (212) 305-7759; Fax: (212) 305-2277; E-mail: firstname.lastname@example.org.
First published September 18, 2008 - More info
Asthma is a complex heritable disease that is increasing in prevalence and severity, particularly in developed countries such as the United States, where 11% of the population is affected. The contribution of environmental and genetic factors to this growing epidemic is currently not well understood. We developed the hypothesis, based on previous literature, that changes in DNA methylation resulting in aberrant gene transcription may enhance the risk of developing allergic airway disease. Our findings indicate that in mice, a maternal diet supplemented with methyl donors enhanced the severity of allergic airway disease that was inherited transgenerationally. Using a genomic approach, we discovered 82 gene-associated loci that were differentially methylated after in utero supplementation with a methyl-rich diet. These methylation changes were associated with decreased transcriptional activity and increased disease severity. Runt-related transcription factor 3 (Runx3), a gene known to negatively regulate allergic airway disease, was found to be excessively methylated, and Runx3 mRNA and protein levels were suppressed in progeny exposed in utero to a high-methylation diet. Moreover, treatment with a demethylating agent increased Runx3 gene transcription, further supporting our claim that a methyl-rich diet can affect methylation status and consequent transcriptional regulation. Our findings indicate that dietary factors can modify the heritable risk of allergic airway disease through epigenetic mechanisms during a vulnerable period of fetal development in mice.
John W. Hollingsworth, Shuichiro Maruoka, Kathy Boon, Stavros Garantziotis, Zhuowei Li, John Tomfohr, Nathaniel Bailey, Erin N. Potts, Gregory Whitehead, David M. Brass, David A. Schwartz
Recently, epigenetic-mediated mechanisms — which involve heritable changes in gene expression in the absence of alterations in DNA sequences — have been proposed as contributing to asthma. In this issue of the JCI, Hollingsworth and colleagues report on the effect of prenatal maternal dietary intake of methyl donors on the risk of allergic airway disease in offspring in mice and show that these effects involve epigenetic regulation (see the related article beginning on page 3462). Supplementation of the maternal diet with methyl donors was associated with greater airway allergic inflammation and IgE production in F1 and, to some extent, F2 progeny. Site-specific differences in DNA methylation and reduced transcriptional activity were detected. If these findings are confirmed, a new paradigm for asthma pathogenesis may be emerging.
More and more, it seems that our traditional view of asthma as a complex disease that is mediated by a genetic predisposition and childhood or later environmental exposures needs updating. At this point, a mounting body of literature has established that prenatal exposures can influence the risk for developing asthma (1). This link has been most firmly documented in epidemiological studies of prenatal exposure to cigarette smoke and subsequent wheeze. For example, in a large prospective Danish cohort study of over 11,000 children, maternal smoking at the 36th week of gestation was associated with transient wheezing in children before age 3 years (2). In a Stockholm cohort of over 4,000 newborns, maternal smoking during but not following pregnancy was associated with an increased risk of recurrent wheezing in offspring up to age two years (3). In mouse models, exposure during pregnancy to an air pollutant aerosol (residual oil fly ash) led to a greater susceptibility to an asthma-like phenotype in the offspring mice (4).
The relationship between a mother’s diet during pregnancy and the child’s subsequent risk of developing asthma or atopy has become a topic of growing investigation. Reduced maternal intake of vitamin E, vitamin D, and zinc during pregnancy all have been associated with a greater risk of development of asthma and wheezing symptoms in 5-year-old children (5, 6). Most recently, Chatzi and colleagues found that adherence to a Mediterranean diet during pregnancy was associated with protection from persistent wheeze and atopy in children (7). Frequent maternal intake of fish during pregnancy also reduced the risk of food and possibly inhalant allergic sensitizations (8). Daily consumption of nut products during pregnancy has been associated with asthma in the child by age 8 years (9). However, in a recent meta-analysis of four clinical studies, avoidance of specific food antigens during pregnancy did not appear to influence the risk of development of atopic disease in the child (10). Combined, these studies suggest that the prenatal diet can alter the intrauterine environment in complex and possibly inconsistent ways.
Current hypotheses tend to consider either immune-mediated or epigenetic-mediated mechanisms. It now appears evident that antigen-specific B cell and T cell immune responses can develop following antigen exposures during pregnancy, as our group has demonstrated following prenatal vaccination against influenza antigens (11). Prenatal environmental exposures may be able to alter the intrauterine cytokine milieu (12). However, proof that prenatal dietary exposure delays or prevents early sensitization to food antigens and hence risk for clinical atopy has so far been more elusive (13). Lately, epigenetic-mediated mechanisms that entail heritable changes in gene expression that occur in the absence of alterations in DNA sequences have been proposed (14). These changes usually involve either DNA methylation (covalent addition of a methyl group to cytosines in CpG dinucleotides) or chromatin packaging of DNA via posttranslational modifications of histones (Figure 1).
Prenatal maternal diet affects asthma risk in offspring. Prenatal maternal environmental exposure to diets high in folate, vitamin B12, choline, and methionine, which provide methyl donors, as well as to cigarette smoking may be associated with the covalent addition of a methyl group to cytosines in CpG dinucleotides and other epigenetic changes. These changes in turn may repress gene transcription and induce asthma phenotypes (i.e., airway allergic inflammation) in the offspring. As reported by Hollingsworth and colleagues in their study in mice in this issue of the JCI (25), prenatal methyl-rich diets may promote DNA methylation and reduce transcription of genes associated with the downregulation of allergic immune responses in the airway, such as runt-related transcription factor 3 (Runx3).
In their seminal studies, Cooney and colleagues and Waterland and Jirtle demonstrated that methyl supplementation of the maternal diet during pregnancy with folic acid, vitamin B12, and other agents increased DNA methylation of the long terminal repeat controlling the expression of the agouti gene and thereby influenced the methylation-dependent phenotype (coat color distribution) in mouse offspring (15, 16). Maternal stress and impaired nurturing in addition to environmental exposures to xenobiotic chemicals, endocrine disrupters, and low-dose radiation all have been associated with epigenetic gene regulation without changing the DNA sequence (reviewed in refs. 17, 18).
Epigenetic regulation also may be inherited across multiple generations, implying that not only prenatal maternal but also grandmaternal exposures may influence subsequent gene expression in the children. Examples of multigenerational effects on phenotype have been demonstrated in studies on the kinked tail (AxinFu) and agouti-viable yellow (Avy) allele, associations between endocrine disrupters and male infertility, and hormone-dependent cancer risk (18). While clearly multigenerational effects of prenatal environmental exposures are difficult to demonstrate in human epidemiological studies, one study is quite suggestive. In a provocative nested case control study by the Children’s Health Study of children diagnosed with asthma by age 5 years versus control subjects, grandmaternal smoking during pregnancy was associated with a greater risk for developing asthma in grandchildren. Borderline statistical significance was achieved when the effect was studied independently of maternal smoking (19).
In the asthma literature, articles supporting epigenetic mechanisms are just beginning to be published. These include mouse studies demonstrating that inhaled diesel exposure and intranasal Aspergillus fumigatus allergen induced hypermethylation at multiple sites of the IFN-γ promoter and hypomethylation at the CpG-408 site of the IL-4 promoter. Altered methylation of both gene promoters was correlated significantly with changes in IgE levels (20). In other recent work, cell-specific DNA methylation at the A disintegrin and metalloprotease 33 (ADAM33) gene promoter, an area whose expression has been implicated in severe asthma, has been reported. In this study, promoter methylation levels differed considerably between epithelial cells and fibroblasts, and this in turn differentially regulated ADAM33 gene expression (21). In another study, bronchial biopsies obtained from untreated asthmatics possessed greater levels of histone acetyltransferase and lower levels of histone deacetylase (HDAC) enzymatic activity. Levels reversed following treatment with inhaled steroids (22). Because, in general, acetylation of histones is associated with gene induction and deacetylation is associated with gene silencing (23), the study suggests that one antiinflammatory mode of action in the pharmacological treatment of asthma may be epigenetic. Finally, trichostatin A–induced inhibition of endogenous HDAC upregulated Th2 cytokine (IL-13, IL-5) and GATA3–mediated CD45RO+ T cell recall responses (24), suggesting that Th polarization can be directly epigenetically regulated.
The study by Hollingsworth and colleagues in this issue of the JCI (25) is the first to report on the effect of dietary methyl donors on the risk for allergic airway disease via epigenetic mechanisms. Using an approach similar to that developed by Waterland and Jirtle when they examined agouti coat color, Hollingsworth’s group fed mice a diet supplemented with methyl donors during gestation and weaning. Supplementation of the maternal diet with methyl donors was associated with greater levels of airway hyperactivity, airway eosinophilic inflammation, chemokines KC, macrophage inflammatory protein 1β (MIP1β), and RANTES, and IgE production in the F1 progeny. There was a less robust effect on airway eosinophilic inflammation and IgE level in the F2 generation mice. Furthermore, the authors profiled site-specific differences in DNA methylation in phenotypic extremes of the F1 progeny that were gestated on either a high- or low-methylation diet. Identifying and focusing on 5 candidate genes in validation gene-expression experiments, they were able to confirm that in utero supplementation with a methyl-rich diet was associated with decreased transcriptional activity and mRNA expression in lung tissue. One of these genes was runt-related transcription factor 3 (Runx3), a gene known to downregulate allergic airway inflammation. Diets supplemented with methyl donors during either lactation or adulthood did not significantly affect the airway disease phenotype of mice, suggesting that the critical time window of susceptibility is gestation. Hence, the authors demonstrated that prenatal diet and methyl donation are associated with altered asthma-related phenotypes across multiple generations via epigenetic mechanisms and also proposed candidate genes and maybe an epigenome that could be (and would need to be) validated in future studies.
One cannot ignore the observation that the increase in asthma prevalence over recent decades approximately coincides with worldwide campaigns that recommend periconceptional dietary folate supplementation (26). From a public health perspective, the adverse nonrespiratory health consequences of insufficient prenatal folate consumption are legitimate concerns. But an even broader public health issue has surfaced. If confirmed, prenatal exposures may influence the development of asthma not only for our children, but for their children as well.
Despite the provocative nature of the findings of the study by Hollingsworth and colleagues (25), they need to be interpreted with serious caution. Mouse models for asthma, being mouse models, are limited in their direct applicability to human asthma. Translation to the clinical arena would require cohort-driven epigenetic research, particularly from studies powered sufficiently to examine questions of dose and timing of prenatal environmental and/or dietary exposure, on the subsequent clinical risk for asthma. Nonetheless, this study seems to have uncovered a new form of environmental exposure — prenatal maternal dietary exposure to methyl groups — that together with certain genetic events may increase the risk of asthma in offspring. Let us hope that our scientific community can design studies to validate these findings in human disease, not only for the benefit of our children, but potentially for their children as well.
The author is supported by grants R21 ES013063, R01 ES013163, and P50ES015905 from the NIH.
Conflict of interest: The author has declared that no conflict of interest exists.
Reference information: J. Clin. Invest.118:3265–3268 (2008). doi:10.1172/JCI37171.
See the related article at In utero supplementation with methyl donors enhances allergic airway disease in mice.