Redox modifier genes in amyotrophic lateral sclerosis in mice
Jennifer J. Marden, Maged M. Harraz, Aislinn J. Williams, Kathryn Nelson, Meihui Luo, Henry Paulson, John F. Engelhardt
Jennifer J. Marden, Maged M. Harraz, Aislinn J. Williams, Kathryn Nelson, Meihui Luo, Henry Paulson, John F. Engelhardt
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Research Article

Redox modifier genes in amyotrophic lateral sclerosis in mice

Abstract

Amyotrophic lateral sclerosis (ALS), one of the most common adult-onset neurodegenerative diseases, has no known cure. Enhanced redox stress and inflammation have been associated with the pathoprogression of ALS through a poorly defined mechanism. Here we determined that dysregulated redox stress in ALS mice caused by NADPH oxidases Nox1 and Nox2 significantly influenced the progression of motor neuron disease caused by mutant SOD1G93A expression. Deletion of either Nox gene significantly slowed disease progression and improved survival. However, 50% survival rates were enhanced significantly more by Nox2 deletion than by Nox1 deletion. Interestingly, female ALS mice containing only 1 active X-linked Nox1 or Nox2 gene also had significantly delayed disease onset, but showed normal disease progression rates. Nox activity in spinal cords from Nox2 heterozygous female ALS mice was approximately 50% that of WT female ALS mice, suggesting that random X-inactivation was not influenced by Nox2 gene deletion. Hence, chimerism with respect to Nox-expressing cells in the spinal cord significantly delayed onset of motor neuron disease in ALS. These studies define what we believe to be new modifier gene targets for treatment of ALS.

Authors

Jennifer J. Marden, Maged M. Harraz, Aislinn J. Williams, Kathryn Nelson, Meihui Luo, Henry Paulson, John F. Engelhardt

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Figure 1

Deletion of NADPH oxidase genes (Nox1 or Nox2) enhances survival and survival index in ALS mice and significantly reduces superoxide production in spinal cords of end-stage SOD1G93A mice.

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Deletion of NADPH oxidase genes (Nox1 or Nox2) enhances survival and sur...
(A) Survival curves for hemizygous SOD1G93A transgenic mice with the indicated Nox1 and Nox2 genotypes. Median 50% survival rates were as follows: Nox1-WT (n = 8), 129 days; Nox1-HET (n = 6), 140 days, P < 0.03 versus Nox1-WT; Nox1-KO (n = 7), 162 days, P < 0.004 versus Nox1-WT; Nox2-WT (n = 16), 132 days; Nox2-HET (n = 8), 186 days, P < 0.0001 versus Nox2-WT; Nox2-KO (n = 24), 229 days, P < 0.0001 versus Nox2-WT. (B) Survival index for mice in A; onset of disease was defined as 10% weight loss from peak weight. Values are mean ± SEM. *P = 0.059, †P < 0.0001 versus WT. (C and D) NADPH-dependent superoxide production in spinal cords of hemizygous SOD1G93A transgenic mice with WT, HET, and KO Nox2 genotypes. The relative mean rate ± SEM of superoxide production is plotted. (C) Ages at the time of spinal cord harvest were as follows: Nox2-WT, 125, 132, and 124 days; Nox2-HET, 186, 179, and 183 days; Nox2-KO, 224, 223, and 242 days (n = 3 per genotype). All mice were at the stage of clinical death at the time of harvest. †P < 0.008, *P < 0.045 versus WT; Student’s t test. (D) All mice were harvested at 120 days of age (n = 4 per genotype). †P < 0.0001 versus WT; Student’s t test.