Time-dependent changes in the density and hemoglobin F content of biotin-labeled sickle cells.

R S Franco; J Lohmann; E B Silberstein; G Mayfield-Pratt; M Palascak; T A Nemeth; C H Joiner; M Weiner; D L Rucknagel

doi:10.1172/JCI2484

University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0508, USA. Robert.Franco@uc.edu

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Published in Volume 101, Issue 12 on June 15, 1998
J Clin Invest. 1998;101(12):2730–2740. https://doi.org/10.1172/JCI2484.
© 1998 The American Society for Clinical Investigation

Published June 15, 1998 - Version history

Sickle red blood cells (RBC) are subject to a number of important cellular changes and selection pressures. In this study, we validated a biotin RBC label by comparison to the standard 51Cr label, and used it to study changes that occur in sickle cells as they age. Sickle RBC had a much shorter lifespan than normal RBC, but the two labels gave equivalent results for each cell type. A variable number of sickle, but not normal, RBC disappeared from the circulation during the first few hours after reinfusion. The number of biotinylated sickle reticulocytes was decreased by 50% after 24 h and 75% after 48 h, with a gradual decrease in the amount of reticulum per cell. The labeled sickle cells exhibited major density increases during the first 4-6 d after reinfusion, with smaller changes thereafter. A small population of very light, labeled sickle RBC was essentially constant in number after the first few days. Fetal hemoglobin (HbF) content was determined in isolated biotinylated sickle RBC after reinfusion, allowing an estimate of lifespan for RBC containing HbF (F cells) and non-F cells. The lifespan of sickle biotinylated RBC lacking HbF was estimated to be approximately 2 wk, whereas F cells survived 6-8 wk.

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Time-dependent changes in the density and hemoglobin F content of biotin-labeled sickle cells.

R S Franco, J Lohmann, E B Silberstein, G Mayfield-Pratt, M Palascak, T A Nemeth, C H Joiner, M Weiner, and D L Rucknagel

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Time-dependent changes in the density and hemoglobin F content of biotin-labeled sickle cells.

R S Franco, J Lohmann, E B Silberstein, G Mayfield-Pratt, M Palascak, T A Nemeth, C H Joiner, M Weiner, and D L Rucknagel

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