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Saturated fat–rich diet enhances selective uptake of LDL cholesteryl esters in the arterial wall
Toru Seo, … , Rajasekhar Ramakrishnan, Richard J. Deckelbaum
Toru Seo, … , Rajasekhar Ramakrishnan, Richard J. Deckelbaum
Published August 1, 2005
Citation Information: J Clin Invest. 2005;115(8):2214-2222. https://doi.org/10.1172/JCI24327.
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Research Article Cardiology

Saturated fat–rich diet enhances selective uptake of LDL cholesteryl esters in the arterial wall

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Abstract

Plasma LDL levels and atherosclerosis both increase on a saturated fat–rich (SAT) diet. LDL cholesterol delivery to tissue may occur via uptake of the LDL particles or via selective uptake (SU), wherein cholesteryl ester (CE) enters cells without concomitant whole-particle uptake. It is not known how dietary fats might directly affect arterial LDL-CE uptake and whether SU is involved. Thus, mice that are relatively atherosclerosis resistant (C57BL/6) or susceptible to atherosclerosis (apoE–/–) were fed a chow or SAT diet and injected with double radiolabeled or fluorescent-labeled human LDL to independently trace LDL-CE core and whole-particle uptake, respectively. Our results show that a SAT diet increased contributions of SU to total arterial LDL-CE delivery in C57BL/6 and apoE–/– mice. The SAT diet increased plasma fatty acid and cholesterol levels; cholesterol, but not fatty acid, levels correlated with SU, as did the degree of atherosclerosis. Increased SU did not correlate with arterial scavenger receptor class B type I levels but paralleled increased lipoprotein lipase (LPL) levels and LPL distribution in the arterial wall. These studies suggest that arterial LDL-CE delivery via SU can be an important mechanism in vivo and that dietary influences on arterial LPL levels and atherogenesis modulate arterial LDL-CE delivery, cholesterol deposition, and SU.

Authors

Toru Seo, Kemin Qi, Chuchun Chang, Ying Liu, Tilla S. Worgall, Rajasekhar Ramakrishnan, Richard J. Deckelbaum

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Figure 7

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Effects of different diets on LPL and SR-BI in the arterial wall in C57B...
Effects of different diets on LPL and SR-BI in the arterial wall in C57BL/6 and apoE–/– mice. Arteries extending from aorta to tracheal artery from C57BL/6 and apoE–/– mice fed a chow or SAT for 12 weeks (n = 3–5) were pooled and homogenized for Western blot analyses for LPL and SR-BI. At least 3 Western blots were performed, and they showed very similar results. Changes in arterial LPL or SR-BI expression induced by SAT relative to chow diet were calculated and expressed as relative density change ± SEM.

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