CD8⁺ Des-TCR T cells activated within the liver exhibited defective CTL function. (A) Representative histograms of CD43 activation–associated glycoform expression by CD8⁺ Des-TCR cells in livers of Met-K^b mice treated with anti-CD62L (thin line) or control IgG (thick line). Organs were harvested 2.5 days after transfer of Des-TCR LN cells. (B) CTL activity of CD8⁺ Des-TCR cells harvested from the livers of Met-K^b mice treated with anti-CD62L (open squares) or control IgG (filled squares) toward P815-K^b target cells at 2.5 days after transfer. The effector/target (E/T) ratio was calculated by determination of the exact number of transgenic T cells present in the well. CTL activity toward P815 control target cells was not detected (data not shown). (C and D) Representative CD43 activation–associated glycoform histograms (C) and CFSE profiles (D) of CD8⁺ Des-TCR cells from the livers of Alb-K^b (H-2K^dk) (thin line) and Met-K^b (H-2K^dk) mice (thick line). Organs were harvested 6 days after transfer of Des-TCR (H-2K^dk) LN cells, and lymphocytes were prepared and analyzed by FACS. All plots were gated on forward and side scatter gates appropriate for lymphocytes and CD8⁺ Des-TCR⁺ PI^– cells. (E) CTL activity of CD8⁺ Des-TCR cells harvested from the livers and LNs of Alb-K^b (H-2K^dk) (round symbols) and Met-K^b (H-2K^dk) mice (square symbols) incubated with P815 (open symbols) or P815-K^b target cells (filled symbols). Organs were harvested 6 days after transfer of Des-TCR (H-2K^dk) LN cells. The effector/target ratio was calculated by determination of the exact number of transgenic T cells present in each well.