Intrahepatic activation of CD8⁺ Des-TCR T cells was associated with reduced half-life. (A and B) Administration of anti-CD62L mAb to Met-K^b mice resulted in reduced CD8⁺ Des-TCR T cell numbers 2.5 and 6 days after adoptive transfer. Unmanipulated and splenectomized Met-K^b and B10.BR mice were preadministered anti-CD62L mAb or purified rat IgG as described in Methods. A further dose of antibodies was administered intraperitoneally 24 hours after adoptive transfer. Organs were harvested at day 2.5 (A) and day 6 (B), and lymphocytes were analyzed by flow cytometry. Cell numbers for blood represent CD8⁺ Des-TCR T cells/ml; cell numbers for other organs represent total CD8⁺ Des-TCR T cells from the organ. Total cell numbers represent the sum of CD8⁺ Des-TCR T cells retrieved from these organs. Plots represent means ± SEM for groups of 3 mice. (C) T cells activated within the liver are committed to reduced half-life within the first 24 hours following activation. Equal numbers of CFSE-labeled Des-TCR LN cells activated for 24 hours in either liver or LNs of Met-K^b mice were adoptively transferred into a second antigen-free, nontransgenic B10.BR recipient, and 30 days later, lymphocytes from liver, LNs, and spleen were purified as described in Methods. Bars indicate the total number of CD8⁺ CFSE⁺ Des-TCR⁺ cells harvested from the 3 compartments. Plots represent means ± SEM for groups of 3 mice.