Administration of anti-CD62L antibody reduced LN entry by CD8⁺ Des-TCR T cells but did not affect their intrahepatic activation or proliferation in Met-K^b mice. (A) Total number of Ly5.1^– CD8⁺ Des-TCR T cells in various organs of Ly5.1⁺ Met-K^b mice pretreated with anti-CD62L mAb or PBS and injected 4 hours later with Ly5.1^– Des-TCR LN cells. Organs were harvested 4 hours after cell transfer. (B) CD69 expression by donor Ly5.1^– CD8⁺ Des-TCR T cells (solid line) versus recipient Ly5.1⁺ CD8⁺ T cells (dotted line) within the livers of Met-K^b mice at 4 hours after adoptive transfer. Representative histograms were gated on forward and side scatter gates appropriate for lymphocytes, and Ly5.1^– CD8⁺ PI– cells for CD8⁺ Des-TCR T cells or Ly5.1⁺ CD8⁺ PI^– cells for liver resident non–Des-TCR CD8⁺ T cells. (C) CFSE profiles of donor CD8⁺ Des-TCR T lymphocytes, at 2.5 days after transfer, isolated from non-splenectomized and splenectomized Met-K^b (shaded plot) and B10.BR (dashed line) mice preadministered control purified rat IgG, or Met-K^b mice preadministered anti-CD62L mAb (solid line). CD8⁺ Des-TCR T cells did not proliferate in the livers of B10.BR mice administered anti-CD62L (data not shown). (D) CFSE profiles of donor CD8⁺ Des-TCR lymphocytes isolated from the liver (solid line) and LNs (shaded plot) of Met-K^b mice administered control rat IgG illustrate that donor T cells activated at these 2 sites proliferated at a similar rate.