Influence of langerin (CD207) on antigen presentation in cord blood–derived LC-like DCs and freshly isolated epidermal LCs. (A) LC-like DCs, preincubated with anti-CD207 antibodies or mouse IgG1, were cocultured with CD1a-restricted T cell clones B2.1 and B2.11 and CD1b-restricted T cells (line DN1) in the presence or absence of antigen (M. leprae sonicate for B2.1 and B2.11, M. tuberculosis sonicate for DN1). Proliferation was measured as described for Figure 2. (B) LC-like DCs were pulsed for 4 hours with antigen (M. leprae sonicate) or with media and cocultured with CD1a-restricted T cells. Anti-CD207 antibodies were added before (anti-CD207 → Ag) or after (Ag → anti-CD207) pulsing of the LC-like DCs with antigen. Proliferation was measured as described for Figure 2. (C) CD1a-restricted T cells (clone B2.11) were cocultured with epidermal cell suspension (EC) or EC depleted of LCs (LC-depl. EC) in the presence or absence of antigen (M. leprae sonicate, 5 μg/ml). Proliferation was measured as described for Figure 2. (D) EC, preincubated with the indicated antibodies or mouse IgG1, was cocultured with CD1a-restricted T cells (clone B2.11) in the presence or absence of antigen. Proliferation was measured as described for Figure 2. The values shown are the mean ± SEM of triplicate cultures and are representative of at least three (A) or two (B–D) independent experiments. Statistical analysis was performed as indicated in the legend to Figure 2, but comparing T cells stimulated with media versus antigen. *P < 0.05; **P < 0.005.