Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • Aging (Upcoming)
    • Next-Generation Sequencing in Medicine (Jun 2022)
    • New Therapeutic Targets in Cardiovascular Diseases (Mar 2022)
    • Immunometabolism (Jan 2022)
    • Circadian Rhythm (Oct 2021)
    • Gut-Brain Axis (Jul 2021)
    • Tumor Microenvironment (Mar 2021)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells
Robert E. Hunger, … , Steven A. Porcelli, Robert L. Modlin
Robert E. Hunger, … , Steven A. Porcelli, Robert L. Modlin
Published March 1, 2004
Citation Information: J Clin Invest. 2004;113(5):701-708. https://doi.org/10.1172/JCI19655.
View: Text | PDF
Article Dermatology

Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells

  • Text
  • PDF
Abstract

Langerhans cells (LCs) constitute a subset of DCs that initiate immune responses in skin. Using leprosy as a model, we investigated whether expression of CD1a and langerin, an LC-specific C-type lectin, imparts a specific functional role to LCs. LC-like DCs and freshly isolated epidermal LCs presented nonpeptide antigens of Mycobacterium leprae to T cell clones derived from a leprosy patient in a CD1a-restricted and langerin-dependent manner. LC-like DCs were more efficient at CD1a-restricted antigen presentation than monocyte-derived DCs. LCs in leprosy lesions coexpress CD1a and langerin, placing LCs in position to efficiently present a subset of antigens to T cells as part of the host response to human infectious disease.

Authors

Robert E. Hunger, Peter A. Sieling, Maria Teresa Ochoa, Makoto Sugaya, Anne E. Burdick, Thomas H. Rea, Patrick J. Brennan, John T. Belisle, Andrew Blauvelt, Steven A. Porcelli, Robert L. Modlin

×

Figure 1

Options: View larger image (or click on image) Download as PowerPoint
Phenotype of cord blood–derived LC-like DCs and MD-DCs. (A) LC-like DCs ...
Phenotype of cord blood–derived LC-like DCs and MD-DCs. (A) LC-like DCs and MD-DCs were stained with mAb’s specific for CD1a (solid line) and mouse IgG control antibody (dashed line). MFI is indicated for the positive cell population. (B) LC-like DCs and MD-DCs were double-stained with mAb’s specific for CD1a and langerin. The numbers indicate the percentage of cells in each quadrant. One representative experiment is shown from four independent donors. (C) LC-like DCs and MD-DCs were stained with numerous surface proteins. MFI was determined by flow cytometry. For each data point, cells from three different donors were analyzed (± SEM). The ratios of MFI from LC-like DCs to MFI from MD-DCs were calculated and shown on a log scale as indicated.

Copyright © 2022 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts