Peripheral blood was collected from patients with hematological malignancy, such as CD19⁺ NHL, and used as the source for T cells in the generation of two CAR-T cell products. Transduction of activated T cells was performed using two distinct vectors encoding the first generation and second-generation CAR.CD19, and CAR-T cells were expanded in parallel. The two CAR-T cells products were then co-infused in the patient, and blood and tumor samples were collected to detect each product. PCR can be used as an assay to monitor the kinetics of each product in patients. CAR-T cells encoding the CD28 signaling motif showed superior expansion and persistence in patients with CD19⁺ NHL (13).