(A) Representative ultrasound images of descending aorta. Magnified views show pulse-wave Doppler mode graphs, with mean velocity in systole indicated with blue dashed lines (below baseline); in progeroid mice, retrograde flow in diastole is detected as a plateau above baseline (blue arrowhead). (B) Percentage of mice with aortic insufficiency (retrograde flow) in the descending aorta: 0 of 10 Lmna^+/+ mice; 5 of 10 Lmna^G609G/G609G mice. (C) Blood flow mean velocity values in the indicated aortic regions (n = 9–10). (D) Pulsatility index and resistive index in abdominal aorta (n = 10). (E) Representative en face immunofluorescence images of thoracic aortae, showing ECs (CD31, green), Golgi apparatus (GLG1, white), and nuclei (Hoechst 33342, blue), and percentage of ECs with mispolarized Golgi apparatus (downstream orientation relative to nuclei, arrowheads) (n = 6). Mean values for each mouse were determined by averaging of values from 3 fields. (F) Representative en face immunofluorescence images of thoracic aortae, showing ECs (CD31, green), primary cilia (ARL13B, white), and nuclei (Hoechst 33342, blue), and percentage of ciliated ECs (arrowheads) (n = 12). Mean values for each mouse were determined by averaging of values from 3 fields. (G and H) Overlap between gene expression changes in progeroid aortic ECs (C6 cluster in Figure 1, Lmna^G609G/G609G vs. Lmna^+/+ mice) and wild-type carotid ECs exposed to disturbed blood flow (carotid partial ligation vs. sham, results reported in ref. 25). Red dots in H highlight YAP/TAZ downstream targets significantly upregulated in both conditions. Data in C–F are shown as mean + SD. Statistical analysis was performed by 2-sided Fisher’s exact test (B) and unpaired 2-tailed Student’s t test (C–F). Outliers assessed by ROUT test in C and D were excluded for analysis. Scale bars: 50 μm. *P < 0.05, **P < 0.01, ****P < 0.0001. CPL, carotid partial ligation; DEG, differentially expressed genes; FC, fold change; GA, Golgi apparatus.