(A and B) Skeletal muscle volume of the limb and fat infiltration in muscle evaluated by in vivo microCT. (C) Maximal speed evaluated on treadmill normalized by gastrocnemius mass. (D) Limb force evaluated by handgrip normalized by gastrocnemius mass (n = 8 per group). (E) Body temperature evaluated by infrared camera (n = 8 per group). (F) Muscle fiber type, number, and area. Note the type I fibers in blue dark and type II fibers in light blue. Original magnification is ×10. (G) ITT AUC (n = 8 per group). (H) GTT. (I) Relative protein expression in the gastrocnemius. Hatch marks correspond to mice that have received an acute injection of insulin. (J) Relative mRNA expression of insulin signaling in soleus. (K) Relative mRNA expression of Nfkb signaling in soleus (n = 6 per group). Statistical differences were assessed by 1-way ANOVA. ^†P < 0.05, ^‡P < 0.01 significant difference versus WT. Bars show mean ± SEM.