Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability
Huixing Wu, … , Kwang Sik Kim, Francis X. McCormack
Huixing Wu, … , Kwang Sik Kim, Francis X. McCormack
Published May 15, 2003
Citation Information: J Clin Invest. 2003;111(10):1589-1602. https://doi.org/10.1172/JCI16889.
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Article Pulmonology

Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability

Abstract

The pulmonary collectins, surfactant proteins A (SP-A) and D (SP-D), have been reported to bind lipopolysaccharide (LPS), opsonize microorganisms, and enhance the clearance of lung pathogens. In this study, we examined the effect of SP-A and SP-D on the growth and viability of Gram-negative bacteria. The pulmonary clearance of Escherichia coli K12 was reduced in SP-A–null mice and was increased in SP-D–overexpressing mice, compared with strain-matched wild-type controls. Purified SP-A and SP-D inhibited bacterial synthetic functions of several, but not all, strains of E. coli, Klebsiella pneumoniae, and Enterobacter aerogenes. In general, rough E. coli strains were more susceptible than smooth strains, and collectin-mediated growth inhibition was partially blocked by coincubation with rough LPS vesicles. Although both SP-A and SP-D agglutinated E. coli K12 in a calcium-dependent manner, microbial growth inhibition was independent of bacterial aggregation. At least part of the antimicrobial activity of SP-A and SP-D was localized to their C-terminal domains using truncated recombinant proteins. Incubation of E. coli K12 with SP-A or SP-D increased bacterial permeability. Deletion of the E. coli OmpA gene from a collectin-resistant smooth E. coli strain enhanced SP-A and SP-D–mediated growth inhibition. These data indicate that SP-A and SP-D are antimicrobial proteins that directly inhibit the proliferation of Gram-negative bacteria in a macrophage- and aggregation-independent manner by increasing the permeability of the microbial cell membrane.

Authors

Huixing Wu, Alexander Kuzmenko, Sijue Wan, Lyndsay Schaffer, Alison Weiss, James H. Fisher, Kwang Sik Kim, Francis X. McCormack

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Figure 8

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Growth inhibition by rSP-A and rSP-D is blocked by J5 LPS. (a) E. coli K...
Growth inhibition by rSP-A and rSP-D is blocked by J5 LPS. (a) E. coli K12 were grown in the presence of hSP-A (50 or 100 μg/ml) or rSP-D (5 μg/ml) and E. coli J5 LPS vesicles (300 μg/ml) for 90 minutes at 37°C. 3H-uridine incorporation was measured as above. Data are mean ± SEM; n = 3–5 per group; *P < 0.01 compared with E. coli alone. (b) E. coli–impregnated agar was prepared as above, and 0.5 μg of rSP-A or rSP-D was added to the well in the presence or absence of E. coli J5 LPS vesicles (100 μg/ml). Control wells with J5 LPS alone are also shown.