Advertisement

Research Letter Open Access | 10.1172/JCI164303

Neutralizing antibody responses in patients hospitalized with SARS-CoV-2 Delta or Omicron infection

Susanne L. Linderman,1,2 Lilin Lai,1,3 Estefany L. Bocangel Gamarra,1,2 Max S.Y. Lau,4 Srilatha Edupuganti,5 Diya Surie,6 Mark W. Tenforde,6 James D. Chappell,7 Nicholas M. Mohr,8 Kevin W. Gibbs,9 Jay S. Steingrub,10 Matthew C. Exline,11 Nathan I. Shapiro,12 Anne E. Frosch,13 Nida Qadir,14 Meredith E. Davis-Gardner,1,3 M. Juliana McElrath,15 Adam S. Lauring,16 Mehul S. Suthar,1,2,3 Manish M. Patel,6 Wesley H. Self,17 and Rafi Ahmed1,2

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Linderman, S. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Lai, L. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Bocangel Gamarra, E. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Lau, M. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Edupuganti, S. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Surie, D. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Tenforde, M. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Chappell, J. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Mohr, N. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Gibbs, K. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Steingrub, J. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Exline, M. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Shapiro, N. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Frosch, A. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Qadir, N. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Davis-Gardner, M. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by McElrath, M. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Lauring, A. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Suthar, M. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Patel, M. in: PubMed | Google Scholar

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Self, W. in: PubMed | Google Scholar |

1Emory Vaccine Center,

2Department of Microbiology and Immunology,

3Department of Pediatrics,

4Department of Biostatistics and Bioinformatics, and

5The Hope Clinic, Emory University, Atlanta, Georgia, USA.

6CDC, Atlanta, Georgia, USA.

7Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

8Department of Emergency Medicine, University of Iowa, Iowa City, Iowa, USA.

9Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

10Department of Medicine, Baystate Medical Center, Springfield, Massachusetts, USA.

11Department of Medicine, The Ohio State University, Columbus, Ohio, USA.

12Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

13Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA.

14Department of Medicine, UCLA, Los Angeles, California, USA.

15Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

16Departments of Medicine and of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.

17Department of Emergency Medicine and Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Find articles by Ahmed, R. in: PubMed | Google Scholar

Published October 18, 2022 - More info

Published in Volume 132, Issue 23 on December 1, 2022
J Clin Invest. 2022;132(23):e164303. https://doi.org/10.1172/JCI164303.
© 2022 Linderman et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published October 18, 2022 - Version history
View PDF

Related article:

Omicron infection elicits a broad neutralizing response in hospitalized patients with COVID-19
Elizabeth M. McNally
Elizabeth M. McNally
Editor's note

To the Editor: Humoral and cellular immune responses contribute to overall protective immunity against SARS-CoV-2, with neutralizing antibody playing a key role in preventing viral infection. This is evident from the large number of Omicron infections occurring in vaccinated and convalescent patients, since antibodies induced after vaccination or infection by the ancestral WA1 strain do not neutralize Omicron variants efficiently (1, 2). These findings have led to the FDA recommendation for inclusion of the Omicron variant in bivalent COVID-19 vaccines. However, issues have been raised about the value of adding Omicron to the vaccine based on data showing only modest differences between antibody responses after booster immunization with Omicron- versus WA1-based vaccines (3, 4). Also, a recent study has shown that booster responses to Omicron infection are affected by previous SARS-CoV-2 infections (5). Thus, having additional information on the types of neutralizing antibody responses induced after infection with different SARS-CoV-2 variants will be helpful in addressing this important issue.

Here we report live virus neutralization titers against WA1 and the Delta and Omicron (lineages BA.1, BA.2, and BA.5) variants in serum samples collected from hospitalized patients infected with SARS-CoV-2 Delta or Omicron strains. Blood samples were collected from 187 patients hospitalized with acute COVID-19 in the period from July 2021 to March 2022 at 8 US hospitals (Supplemental Methods; supplemental material available online with this article; https://doi.org/10.1172/JCI164303DS1). Of these patients, 26% were immunocompromised (details are shown in Supplemental Table 1). The majority (69%) of the patients were sequence-confirmed for Delta or Omicron infection, and the remaining were classified according to the variant that was dominant at the time of infection. Patients were unvaccinated (n = 80) or vaccinated with COVID-19 mRNA (n = 100) or adenovirus vector (n = 7) vaccine before infection.

In unvaccinated Delta-infected patients, the neutralizing antibody response was highly biased toward the infecting Delta strain, with lower titers against WA1 (6-fold) and strikingly lower titers against BA.1 (60-fold) and BA.2 (22-fold) (Figure 1A). In vaccinated Delta-infected patients, neutralization titers against Delta and WA1 were similar, but again were much lower against BA.1 (17-fold) and BA.2 (9-fold) (Figure 1B). Thus, both unvaccinated and vaccinated Delta-infected patients had significantly lower neutralizing antibody responses to Omicron (as determined by Wilcoxon’s rank sum test). A strikingly different pattern was seen in Omicron-infected patients irrespective of their vaccination history, with a more favorable neutralizing antibody response to BA.1 and BA.2. While BA.1- and BA.2-neutralizing titers were modestly lower in vaccinated compared with unvaccinated Omicron-infected patients, there was a clear trend toward a broader and more balanced antibody response, with similar neutralization titers in response to Delta, BA.1, and BA.2 (Figure 1, C and D). Importantly, the ratio of BA.1- to WA1-neutralizing titers was significantly higher in Omicron- compared with Delta-infected patients in both the unvaccinated (19-fold) and vaccinated (11-fold) groups (Figure 1, E and F). This analysis of the ratio of neutralizing antibody between Omicron versus WA1 within a given individual shows that Omicron infection elicits Omicron-specific antibody responses. These Omicron-neutralizing antibodies could have emerged from either de novo naive B cells or cross-reactive memory B cells.

Live virus–neutralizing antibody titers in hospitalized patients infected wFigure 1

Live virus–neutralizing antibody titers in hospitalized patients infected with SARS-CoV-2 Delta or Omicron variants. In vitro neutralization titers against live WA1, Delta, BA.1, or BA.2 variants in unvaccinated (A and C) and vaccinated (B and D) hospitalized patients infected with a Delta (A and B) or Omicron (C and D) strain. Solid lines indicate sequence-confirmed infections; dashed lines, non-sequence-confirmed; red indicates immunocompromised patients. The ratio of BA.1- to WA1-neutralizing titers in unvaccinated (E) or vaccinated (F) patients. Geometric mean and 95% CI are indicated. For a subset of Omicron-infected patients who were unvaccinated (G) or vaccinated (H), BA.5 neutralization was also assessed. Values above the panels indicate fold differences between groups (P value). P values were calculated by Wilcoxon’s rank sum test.

Given the current dominance of the BA.5 strain, we then tested neutralization against BA.5. Note that our samples were collected prior to the dominance of BA.5, and most of the Omicron-infected patients in our study had been infected with BA.1 (Supplemental Table 1). In the subset of Omicron patient samples that we analyzed, there was detectable neutralization of BA.5, but it was lower than for BA.1. and BA.2 in both vaccinated and unvaccinated patients (Figure 1, G and H, and Supplemental Figure 1). This reduced neutralization activity against BA.5 in patients infected with BA.1 suggests that it is better to have BA.5 than BA.1 in the vaccine.

In summary, our results show that Omicron infection of unvaccinated or vaccinated patients induces a more proportional and balanced neutralizing antibody response to Omicron variants, supporting the recent decision to include Omicron in the bivalent SARS-CoV-2 vaccine. However, it remains to be seen how these findings from infection will translate to vaccination. Our results are from hospitalized patients with high levels of infected cells and antigen that would have efficiently induced a primary response to Omicron in addition to selectively recruiting Omicron-reactive memory B cells. It is possible that a single immunization with the Omicron bivalent vaccine may not be sufficient and that 2 doses may be needed to achieve the desired antibody response (6). Future studies should address this issue, and it will also be interesting to see how the bivalent vaccine works in people who have already been infected with an Omicron strain.

Supplemental material

View Supplemental data

Acknowledgments

The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the CDC.

Address correspondence to: Rafi Ahmed, Emory Vaccine Center, Emory University, Rollins Research Center, Atlanta, Georgia 30329, USA. Email: rahmed@emory.edu.

Footnotes

Conflict of interest: The authors have declared that no conflict of interest exists.

Copyright: © 2022, Linderman et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.

Reference information: J Clin Invest. 2022;132(23):e164303.https://doi.org/10.1172/JCI164303.

See the related Editor's Note at Omicron infection elicits a broad neutralizing response in hospitalized patients with COVID-19.

References
  1. Altarawneh HN, et al. Protection against the Omicron variant from previous SARS-CoV-2 infection. N Engl J Med. 2022;386(13):1288–1290.
    View this article via: CrossRef PubMed Google Scholar
  2. Pajon R, et al. SARS-CoV-2 Omicron variant neutralization after mRNA-1273 booster vaccination. N Engl J Med. 2022;386(11):1088–1091.
    View this article via: CrossRef PubMed Google Scholar
  3. Gagne M, et al. mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits similar B cell expansion, neutralizing responses, and protection from Omicron. Cell. 2022;185(9):1556–1571.
    View this article via: CrossRef PubMed Google Scholar
  4. Moore JPO, Paul A. FDA: Don’t rush a move to change the Covid-19 vaccine composition. STAT News. June 29, 2022. https://www.statnews.com/2022/06/29/fda-dont-rush-to-change-covid-19-vaccine-composition/.
  5. Reynolds CJ, et al. Immune boosting by B.1.1.529 (Omicron) depends on previous SARS-CoV-2 exposure. Science. 2022;377(6603):eabq1841.
    View this article via: CrossRef PubMed Google Scholar
  6. Ellebedy AH, et al. Adjuvanted H5N1 influenza vaccine enhances both cross-reactive memory B cell and strain-specific naive B cell responses in humans. Proc Natl Acad Sci U S A. 2020;117(30):17957–17964.
    View this article via: CrossRef PubMed Google Scholar
Version history
  • Version 1 (October 18, 2022): In-Press Preview
  • Version 2 (December 1, 2022): Electronic publication
Advertisement
Advertisement