Multiepitope CD8+ T cell response to a NY-ESO-1 peptide vaccine results in imprecise tumor targeting
Valérie Dutoit, Robert N. Taub, Kyriakos P. Papadopoulos, Susan Talbot, Mary-Louise Keohan, Michelle Brehm, Sacha Gnjatic, Paul E. Harris, Brygida Bisikirska, Philippe Guillaume, Jean-Charles Cerottini, Charles S. Hesdorffer, Lloyd J. Old, Danila Valmori
Valérie Dutoit, Robert N. Taub, Kyriakos P. Papadopoulos, Susan Talbot, Mary-Louise Keohan, Michelle Brehm, Sacha Gnjatic, Paul E. Harris, Brygida Bisikirska, Philippe Guillaume, Jean-Charles Cerottini, Charles S. Hesdorffer, Lloyd J. Old, Danila Valmori
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Article Immunology

Multiepitope CD8+ T cell response to a NY-ESO-1 peptide vaccine results in imprecise tumor targeting

Abstract

Research Article

Authors

Valérie Dutoit, Robert N. Taub, Kyriakos P. Papadopoulos, Susan Talbot, Mary-Louise Keohan, Michelle Brehm, Sacha Gnjatic, Paul E. Harris, Brygida Bisikirska, Philippe Guillaume, Jean-Charles Cerottini, Charles S. Hesdorffer, Lloyd J. Old, Danila Valmori

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Sarcoma patients vaccinated with NY-ESO-1 peptides 157-165 and 157-167 r...
Sarcoma patients vaccinated with NY-ESO-1 peptides 157-165 and 157-167 respond to distinct overlapping A2-restricted epitopes in the 157-167 region. Polyclonal monospecific CD8+ multimer+ populations were isolated by cell sorting with the corresponding multimers and further expanded in vitro by mitogen-driven stimulation. After 2 weeks of in vitro expansion, each population was stained with multimers incorporating each of the overlapping 9-mer and 10-mer in the 157-167 region.