Wouter J. de Jonge, Karin L. Kwikkers, Anje A. te Velde, Sander J.H. van Deventer, Martijn A. Nolte, Reina E. Mebius, Jan M. Ruijter, Marinus C. Lamers, Wouter H. Lamers
Flow cytometric analysis of bone marrow B-lineage cells of 3-week-old wild-type and F/A-2+/+ mice. B-lineage lymphocytes contained within the red ovals of the upper panels were analyzed for the expression of IgM and CD45R (B220) (middle panels), and the B220-positive, IgM-negative cells were analyzed for the expression of CD24 and CD43 (S7) (lower panels). The relative size of the indicated subpopulations is shown as a fraction of the total number of cells (upper panels), as a fraction of cells in the B-lineage cell window (middle panels), and as a fraction of the B220-positive, IgM-negative cells in the lower panels. The ratio of pre– to pro–B cells is also given in the lower panels. The small squares schematically show the analyzed subpopulations: M, mature B cells; T1, transitional type I B cells; I, immature B cells; P, pre– and pro–B cells; O, other cell types.