Immunohistochemistry/FISH analysis of serial sections from muscle biopsies of DMD-BMT1 (a–c) and D1 (d–f). Numbers in panels a–c identify specific myofibers in serial sections; 7 and 7′ represent a split myofiber. (a–f) Tissue sections stained with different dystrophin Ab’s show the presence of dystrophin-positive myofibers (green signal). (g–i) Four-color FISH/immunohistochemistry analysis for detection of fused diploid donor nuclei in DMD-BMT1 muscle tissue sections. DMD-BMT1 (g and h) and unaffected control female (i) tissue sections stained for dystrophin (magenta) and then hybridized by FISH with an X-centromeric probe (green) and a dystrophin exon 45 probe (red). Nuclei are counterstained in blue by DAPI. ex, exon.