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Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation
Emanuela Gussoni, … , Louis M. Kunkel, Kenneth Weinberg
Emanuela Gussoni, … , Louis M. Kunkel, Kenneth Weinberg
Published September 15, 2002
Citation Information: J Clin Invest. 2002;110(6):807-814. https://doi.org/10.1172/JCI16098.
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Article Stem cells

Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation

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Abstract

Research Article

Authors

Emanuela Gussoni, Richard R. Bennett, Kristina R. Muskiewicz, Todd Meyerrose, Jan A. Nolta, Irene Gilgoff, James Stein, Yiu-mo Chan, Hart G. Lidov, Carsten G. Bönnemann, Arpad von Moers, Glenn E. Morris, Johan T. den Dunnen, Jeffrey S. Chamberlain, Louis M. Kunkel, Kenneth Weinberg

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Figure 3

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Immunohistochemistry/FISH analysis of serial sections from muscle biopsi...
Immunohistochemistry/FISH analysis of serial sections from muscle biopsies of DMD-BMT1 (a–c) and D1 (d–f). Numbers in panels a–c identify specific myofibers in serial sections; 7 and 7′ represent a split myofiber. (a–f) Tissue sections stained with different dystrophin Ab’s show the presence of dystrophin-positive myofibers (green signal). (g–i) Four-color FISH/immunohistochemistry analysis for detection of fused diploid donor nuclei in DMD-BMT1 muscle tissue sections. DMD-BMT1 (g and h) and unaffected control female (i) tissue sections stained for dystrophin (magenta) and then hybridized by FISH with an X-centromeric probe (green) and a dystrophin exon 45 probe (red). Nuclei are counterstained in blue by DAPI. ex, exon.
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