Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation
Emanuela Gussoni, Richard R. Bennett, Kristina R. Muskiewicz, Todd Meyerrose, Jan A. Nolta, Irene Gilgoff, James Stein, Yiu-mo Chan, Hart G. Lidov, Carsten G. Bönnemann, Arpad von Moers, Glenn E. Morris, Johan T. den Dunnen, Jeffrey S. Chamberlain, Louis M. Kunkel, Kenneth Weinberg
Emanuela Gussoni, Richard R. Bennett, Kristina R. Muskiewicz, Todd Meyerrose, Jan A. Nolta, Irene Gilgoff, James Stein, Yiu-mo Chan, Hart G. Lidov, Carsten G. Bönnemann, Arpad von Moers, Glenn E. Morris, Johan T. den Dunnen, Jeffrey S. Chamberlain, Louis M. Kunkel, Kenneth Weinberg
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Article

Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation

Abstract

Research Article

Authors

Emanuela Gussoni, Richard R. Bennett, Kristina R. Muskiewicz, Todd Meyerrose, Jan A. Nolta, Irene Gilgoff, James Stein, Yiu-mo Chan, Hart G. Lidov, Carsten G. Bönnemann, Arpad von Moers, Glenn E. Morris, Johan T. den Dunnen, Jeffrey S. Chamberlain, Louis M. Kunkel, Kenneth Weinberg

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Immunohistochemistry/FISH analysis of serial sections from muscle biopsi...
Immunohistochemistry/FISH analysis of serial sections from muscle biopsies of DMD-BMT1 (a–c) and D1 (d–f). Numbers in panels a–c identify specific myofibers in serial sections; 7 and 7′ represent a split myofiber. (a–f) Tissue sections stained with different dystrophin Ab’s show the presence of dystrophin-positive myofibers (green signal). (g–i) Four-color FISH/immunohistochemistry analysis for detection of fused diploid donor nuclei in DMD-BMT1 muscle tissue sections. DMD-BMT1 (g and h) and unaffected control female (i) tissue sections stained for dystrophin (magenta) and then hybridized by FISH with an X-centromeric probe (green) and a dystrophin exon 45 probe (red). Nuclei are counterstained in blue by DAPI. ex, exon.