Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • Aging (Upcoming)
    • Next-Generation Sequencing in Medicine (Jun 2022)
    • New Therapeutic Targets in Cardiovascular Diseases (Mar 2022)
    • Immunometabolism (Jan 2022)
    • Circadian Rhythm (Oct 2021)
    • Gut-Brain Axis (Jul 2021)
    • Tumor Microenvironment (Mar 2021)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
Live-attenuated virus vaccines for respiratory syncytial and parainfluenza viruses: applications of reverse genetics
Brian R. Murphy, Peter L. Collins
Brian R. Murphy, Peter L. Collins
Published July 1, 2002
Citation Information: J Clin Invest. 2002;110(1):21-27. https://doi.org/10.1172/JCI16077.
View: Text | PDF
Perspective

Live-attenuated virus vaccines for respiratory syncytial and parainfluenza viruses: applications of reverse genetics

  • Text
  • PDF
Abstract

Perspective

Authors

Brian R. Murphy, Peter L. Collins

×

Figure 1

Options: View larger image (or click on image) Download as PowerPoint
Map of the RSV genome and spectrum of attenuation exhibited by gene-dele...
Map of the RSV genome and spectrum of attenuation exhibited by gene-deletion viruses. (a) Map of the RSV genome, a single negative-sense RNA of 15.2 kb. Each viral gene is represented by a box, and the gene-start and gene-end transcription signals that flank each gene are indicated by gray and black bars, respectively. The 3′ and 5′ ends of the genome consist of the extragenic leader (Le) and trailer (Tr) regions, respectively. The genes are separated by short intergenic regions except in the case of the M2 and L genes, which overlap but are nonetheless transcribed into separate mRNAs, as are all of the other genes (1). The diagram is only approximately to scale. (b) Mean peak virus titers in the upper (nasopharynx) and lower (tracheal lavage) respiratory tract of chimpanzees that had been inoculated by the intranasal and intratracheal routes simultaneously with the indicated gene-deletion virus or, as controls, with wild-type RSV or with the rRSVcpts248/404 virus. This last virus serves as a reference point for a virus with mild residual virulence for seronegative infants (4).

Copyright © 2022 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts