Immunolocalization of ABCG5 and ABCG8 in CRL-1601 cells by electron microscopy. Cells were cultured in 100-mm dishes and fixed and processed for immunoelectron microscopy as described in Methods. Localization of ABCG5 (a and b) and ABCG8 (c) in CRL-1601 cells containing empty plasmids (a) or ABCG5 and ABCG8 (b and c). Scale bar = 2 μm.