Expression of Δp85 in the liver of mice injected with an adenoviral vector. (a) Time course of the expression of Δp85 in the liver. The liver was removed from mice at the indicated times after injection with AxCAΔp85, or from uninjected animals (day 0), and was subjected to immunoblot analysis (IB) with an mAb specific for bovine p85. (b) Total homogenates of the indicated tissues obtained from mice 3 days after injection with AxCAΔp85 were subjected to immunoblot analysis with the mAb to bovine p85. (c) Liver homogenates prepared from mice 3 days after injection with AxCALacZ or AxCAΔp85 were subjected to immunoblot analysis with polyclonal antibodies that recognize both rodent and bovine p85. Data in a and b are representative of results obtained from three mice; those in c were obtained from four mice for each group.