SARS-CoV-2 infection and vaccination trigger long-lived B and CD4+ T lymphocytes with implications for booster strategies
Alessio Mazzoni, … , Gian Maria Rossolini, Francesco Annunziato
Alessio Mazzoni, … , Gian Maria Rossolini, Francesco Annunziato
Published February 9, 2022
Citation Information: J Clin Invest. 2022;132(6):e157990. https://doi.org/10.1172/JCI157990.
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Clinical Research and Public Health

SARS-CoV-2 infection and vaccination trigger long-lived B and CD4+ T lymphocytes with implications for booster strategies

Abstract

BACKGROUND Immunization against SARS-CoV-2, the causative agent of COVID-19, occurs via natural infection or vaccination. However, it is currently unknown how long infection- or vaccination-induced immunological memory will last.METHODS We performed a longitudinal evaluation of immunological memory to SARS-CoV-2 up to 1 year after infection and following mRNA vaccination in naive individuals and individuals recovered from COVID-19 infection.RESULTS We found that memory cells are still detectable 8 months after vaccination, while antibody levels decline significantly, especially in naive individuals. We also found that a booster injection is efficacious in reactivating immunological memory to spike protein in naive individuals, whereas it was ineffective in previously SARS-CoV-2–infected individuals. Finally, we observed a similar kinetics of decay of humoral and cellular immunity to SARS-CoV-2 up to 1 year following natural infection in a cohort of unvaccinated individuals.CONCLUSION Short-term persistence of humoral immunity, together with the reduced neutralization capacity versus the currently prevailing SARS-CoV-2 variants, may account for reinfections and breakthrough infections. Long-lived memory B and CD4+ T cells may protect from severe disease development. In naive individuals, a booster dose restored optimal anti-spike immunity, whereas the needs for vaccinated individuals who have recovered from COVID-19 have yet to be defined.FUNDING This study was supported by funds to the Department of Experimental and Clinical Medicine, University of Florence (Project Excellence Departments 2018–2022), the University of Florence (project RICTD2122), the Italian Ministry of Health (COVID-2020-12371849), and the region of Tuscany (TagSARS CoV 2).

Authors

Alessio Mazzoni, Anna Vanni, Michele Spinicci, Giulia Lamacchia, Seble Tekle Kiros, Arianna Rocca, Manuela Capone, Nicoletta Di Lauria, Lorenzo Salvati, Alberto Carnasciali, Elisabetta Mantengoli, Parham Farahvachi, Lorenzo Zammarchi, Filippo Lagi, Maria Grazia Colao, Francesco Liotta, Lorenzo Cosmi, Laura Maggi, Alessandro Bartoloni, Gian Maria Rossolini, Francesco Annunziato

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Figure 6

Reactivation of humoral and cellular immunity following vaccine booster administration in naive individuals and individuals who have recovered from COVID-19.

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Reactivation of humoral and cellular immunity following vaccine booster ...
Levels of anti-spike IgG (A), anti-RBD IgG (B), neutralizing Ig (C) in 7 naive individuals (red) and 7 individuals who have recovered from COVID-19 (blue) before (pre) and 1 week after (post) vaccine booster injection. Levels of spike-specific B (D) and CD4+ T (E) (defined as CD154+ and producing at least 1 cytokine among IFN-γ+, IL-2, and TNF-α cells) in 7 naive individuals (red) and 7 individuals who have recovered from COVID-19 (blue) before and 1 week after vaccine booster injection. Red and blue asterisks denote paired statistics within each study calculated with Wilcoxon’s signed-rank test. Black asterisks (right) represent unpaired statistics between naive individuals and individuals who have recovered from COVID-19 calculated with Mann-Whitney test. *P < 0.05; **P < 0.01