Expression profile of inflamed spinal cords. RNA was isolated as described from inflamed spinal cords 24 days after immunization. Real-time RT-PCR analysis was performed by measuring amplicon accumulation using SYBR Green dye (white bars, WT; black bars, p35^–/–). Data are expressed as arbitrary units based on the standard curve of triplicate wells ± SD. Values shown are representative of at least three individual experiments. Levels of β-actin mRNA were similar in both samples; other mRNA species levels were normalized to β-actin.