Felix Kreier, Eric Fliers, Peter J. Voshol, Corbert G. Van Eden, Louis M. Havekes, Andries Kalsbeek, Caroline L. Van Heijningen, Arja A. Sluiter, Thomas C. Mettenleiter, Johannes A. Romijn, Hans P. Sauerwein, Ruud M. Buijs
Somatotopic organization of the sympathetic nervous system. Sympathetic motor neurons project into one fat compartment only (subcutaneous or intra-abdominal). Two strains of PRV were injected simultaneously into the intra-abdominal fat compartment (mesenterial fat) and the subcutaneous fat compartment (subcutaneous inguinal fat). Confocal laser scanning photomicrograph of transverse thoracic spinal cord sections (Th5–Th10). Both tracers were transported back to the IML and show clear separation of the different tracers (red/green). Insert (control): Injection of both tracers into the same mesenterial fat pad resulted in colocalization of the two tracers (yellow). Specific laser analysis of the indicated neuron (arrow) also showed colocalization of the tracers with a strong signal of FITC (green) and a much weaker signal of CY3 (red). Bar = 50 μm.