Hormone mRNA expression in adipose tissue after parasympathetic denervation. The left retroperitoneal fat pad was parasympathetically denervated (n = 9) and compared with the right intact pad for the expression of mRNA of resistin, leptin, adiponectin, and elongation factor–1α (as a reference gene) by means of real-time RT-PCR. Sham-operated animals were used as control (n = 5). While resistin and leptin mRNA expression was reduced (–71%, Mann-Whitney U test, *P = 0.001; –45%, Mann-Whitney U test, **P = 0.004, respectively), adiponectin and reference mRNA did not change significantly. Thus, parasympathetic denervation of adipose tissue specifically changes mRNA expression of fat-derived hormones. One relative unit is the equivalent cDNA corresponding with 0.1 μg per well of the pooled cDNA of the control fat pads. Values are expressed as mean ± SEM.