AR localizes to keratinocytes, inflammatory cells, and fibroblasts during wound healing. (a) Normal skin immunostaining for AR (left panel) at low magnification (×20) illustrates epidermal and hair follicle staining. Right panels show high magnification (×100) of basal epidermal cells (area 1) and hair follicles (area 2). (b) Day 3 wounds: left panel is a low-magnification image of AR-stained tissue. Area 2 is a high-magnification image of macrophages, and area 1 shows basal and suprabasal epithelial staining at the migrating edge. (c) A day-14 wound (left panel, low magnification) with cells morphologically resembling fibroblasts staining positively for AR; (area 1, right panel) is a high-magnification image. Images are representative of ten wounds stained per timepoint from male mice. (d) AR-positive cells were quantified from day 1 to day 21 after wounding; those colocalizing with Mac-3 were quantified separately. (e) RT-PCR for AR and the housekeeping gene HPRT in wound tissue showed a temporal increase in expression through day 3, with a decrease by day 21. d0, normal, unwounded skin. Bottom panel shows a Western blot for AR (110 kDa) demonstrating an increase in protein levels from basal (d0) to day 7, then a decline in protein to day 21 after wounding. Blot is representative of three experiments using five mice per timepoint.