Peer Wulff, Volker Vallon, Dan Yang Huang, Harald Völkl, Fang Yu, Kerstin Richter, Martina Jansen, Michaela Schlünz, Karin Klingel, Johannes Loffing, Gunther Kauselmann, Michael R. Bösl, Florian Lang, Dietmar Kuhl
Amiloride-sensitive transepithelial potential difference is reduced and αENaC is present in the luminal cell membrane of the renal collecting system of sgk1–/– mice on a low-NaCl diet. (a) Transepithelial potential difference (PD) in isolated perfused collecting ducts at day 3 after initiating a low-NaCl diet (0 g Na+/kg) in the absence (n = 12–16 ducts in 3–5 mice) (white bar) and presence (n = 4–5 ducts in 3–5 mice) (black bar) of 10 μM amiloride. *P < 0.05 versus sgk1+/+ mice. (b) Immunohistochemical localization of αENaC in renal connecting tubule profiles at day 3 after initiating a low-NaCl diet (0 g Na+/kg). Arrowheads indicate apical localization of αENaC.