Mark W. Musch, Lane L. Clarke, Daniel Mamah, Lara R. Gawenis, Zheng Zhang, William Ellsworth, David Shalowitz, Navdha Mittal, Petros Efthimiou, Ziad Alnadjim, Steve D. Hurst, Eugene B. Chang, Terrence A. Barrett
Effect of anti-CD3 mAb on Na+ absorption and anion secretion across murine jejunum. Mice were injected with PBS (Sham-injected) or 0.2 mg anti-CD3 mAb 3 hours before sacrifice. Jejunal sections were mounted in Ussing chambers for measurement of Isc and 22Na flux measurements. (a) Mucosal-to-serosal 22Na flux sensitive to 100 μM EIPA (n = 10 sections from six mice). (b) Isc response to addition of 10 mM glucose in mucosal bath (15 minutes) to stimulate Na+-coupled glucose transport (n = 12 sections from six mice). (c) Isc response to addition of 10 μM forskolin in bathing solutions (10 minutes) to stimulate cAMP-dependent anion secretion (n = 11 sections from six mice). Values are means ± SE. *P < 0.05, **P < 0.1 vs. sham-injected.