IL-7 inhibits OPG production in ROS 17/2.8 cells. (a) Using semiquantitative RT-PCR, we measured OPG mRNA at 24 hours in ROS 17/2.8 cells stimulated with vehicle (PBS plus 0.1% BSA) or IL-7 (10 ng/ml). Data are representative of two independent experiments. (b) OPG mRNA bands in a were quantitated by densitometry and normalized against GAPDH. (c) OPG protein production was measured by Western blot at 24 hours in primary calvarial OBs stimulated with vehicle (PBS plus 0.1% BSA) or IL-7 (10 ng/ml). Data are representative of two independent experiments. (d) OPG protein concentrations in c were quantitated by densitometry.