Effect of a single turpentine injection on serum iron content in wild-type and hepcidin-deficient mice. (a) Serum iron in wild-type mice before (white bar) and after (gray bar) a 16-hour turpentine injection. Results are expressed as mean ± SEM for eight animals. Statistical analysis was performed using Student t test (unpaired, two-tailed). *P < 0.0001. (b) Serum iron in hepcidin-deficient mice before (white bar) and after (gray bar) a 16-hour turpentive injection. Results are expressed as mean ± SEM for seven animals.