Absence of KATP current in aortic smooth muscle cells isolated from Sur2–/– mice. (a) Examples of original whole cell current traces recorded in a Sur2+/+ and a Sur2–/– aortic smooth muscle cell 2 minutes after cell rupture (maximal potassium channel current [IK], left), after 20 μM of glibenclamide (GLB, middle), and the GLB-sensitive IKATP obtained by subtraction (right). The glibenclamide-sensitive current was interpreted as KATP current and was normalized by cell capacitance to obtain the whole cell current density. The GLB-sensitive current was absent from the Sur2–/– cell. (b) Whole cell current protocol. The cell was held at –70 mV, and 235 ms test pulses from –90 to 10 mV were delivered to induce a whole cell current. (c) Mean summary data of the current voltage plot for the GLB-sensitive IKATP density at all voltages tested (n = 13 cells from five Sur2+/+ mice vs. n = 14 cells from four Sur2–/– mice, *P < 0.05).