JCI - Anti-peptide autoantibodies and fatal anaphylaxis in NOD mice in response to insulin self-peptides B:9-23 and B:13-23

Anti-peptide autoantibodies and fatal anaphylaxis in NOD mice in response to insulin self-peptides B:9-23 and B:13-23

Edwin Liu, Hiroaki Moriyama, Norio Abiru, Dongmei Miao, Liping Yu, Robert M. Taylor, Fred D. Finkelman, George S. Eisenbarth

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Article Immunology

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IgG1 antibodies to B:9-23 are detectable within 3 weeks of weekly B:9-23 immunization (100 μg/dose) and peak 2–3 weeks prior to anaphylaxis, in NOD mice. Anaphylaxis occurred at 12 weeks of age in this experiment. Serum obtained from mice is diluted 1:30,000 prior to measurement (lower limit of detection is 1:10⁶ dilution). Various symbols represent the anti–B:9-23 antibody level in individual mice followed over multiple injections. Arrows represent a subcutaneous injection of B:9-23 peptide.

Anti-peptide autoantibodies and fatal anaphylaxis in NOD mice in response to insulin self-peptides B:9-23 and B:13-23

Anti-peptide autoantibodies and fatal anaphylaxis in NOD mice in response to insulin self-peptides B:9-23 and B:13-23

Abstract

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