Craniosynostosis in transgenic mice overexpressing Nell-1
Xinli Zhang, … , Naoaki Saito, Kang Ting
Xinli Zhang, … , Naoaki Saito, Kang Ting
Published September 15, 2002
Citation Information: J Clin Invest. 2002;110(6):861-870. https://doi.org/10.1172/JCI15375.
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Article Genetics

Craniosynostosis in transgenic mice overexpressing Nell-1

Abstract

Research Article

Authors

Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting

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Figure 6

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Effect of Nell-1 downregulation on alkaline phosphatase expression and b...
Effect of Nell-1 downregulation on alkaline phosphatase expression and bone marker expression. (a) Western blot analysis of Nell-1 protein expression in rat FRCCs infected with 20 pfu/cell AdAntiNell-1 or Adβ-Gal control. Downregulation of approximately 60% is observed. (b) Alkaline phosphatase staining (in red) of FRCCs. AdAntiNell-1–infected cells have significantly less staining than do control and AdNell-1–infected cells. (c) Northern analyses of FRCCs on days 3, 6, 9, and 12 after infection. AdAntiNell-1–infected cells have significantly less osteocalcin and osteopontin expression. (d) Expression of osteocalcin (OC) and osteopontin (OP) measured by PhosphorImager and normalized by GAPDH.