Craniosynostosis in transgenic mice overexpressing Nell-1
Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting
Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting
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Article Genetics

Craniosynostosis in transgenic mice overexpressing Nell-1

Abstract

Research Article

Authors

Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting

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Effect of Nell-1 downregulation on alkaline phosphatase expression and b...
Effect of Nell-1 downregulation on alkaline phosphatase expression and bone marker expression. (a) Western blot analysis of Nell-1 protein expression in rat FRCCs infected with 20 pfu/cell AdAntiNell-1 or Adβ-Gal control. Downregulation of approximately 60% is observed. (b) Alkaline phosphatase staining (in red) of FRCCs. AdAntiNell-1–infected cells have significantly less staining than do control and AdNell-1–infected cells. (c) Northern analyses of FRCCs on days 3, 6, 9, and 12 after infection. AdAntiNell-1–infected cells have significantly less osteocalcin and osteopontin expression. (d) Expression of osteocalcin (OC) and osteopontin (OP) measured by PhosphorImager and normalized by GAPDH.