JCI - Hepatocyte-specific inhibition of NF-κB leads to apoptosis after TNF treatment, but not after partial hepatectomy

Hepatocyte-specific inhibition of NF-κB leads to apoptosis after TNF treatment, but not after partial hepatectomy

Michelle L. Chaisson, John T. Brooling, Warren Ladiges, Sophia Tsai, Nelson Fausto

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Inhibition of TNF-induced p65 nuclear translocation in hepatocytes of double-transgenic mice expressing ΔN-IκBα. Liver sections were stained with an antibody to the p65 subunit of NF-κB as described in Methods. (a) Negative control: no primary staining of a single-transgenic mouse killed 3 hours after injection with 5 mg/kg mifepristone. (b) Same tissue stained with p65 antibody. (c and d) p65 staining of a mifepristone-treated single-transgenic mouse (c) or double-transgenic mouse (d) killed 30 minutes after injection of 25 μg/kg TNF. Open arrow indicates an NPC with nuclear p65 staining. Filled arrow indicates a hepatocyte in which p65 nuclear translocation has been blocked. Original magnification, ×400.

Hepatocyte-specific inhibition of NF-κB leads to apoptosis after TNF treatment, but not after partial hepatectomy

Hepatocyte-specific inhibition of NF-κB leads to apoptosis after TNF treatment, but not after partial hepatectomy

Abstract

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Figure 2

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