Effect of melphalan, alone (a) or in combination with NGR-mTNF (c) or mTNF (d), on the tumor growth (a–d) and body weight (e and f) of mice bearing B16F1 melanoma. The animals were treated intraperitoneally with the drugs and the doses indicated in each panel (five animals per group) at days 4, 7, and 9 after tumor implantation (indicated by arrows).