Groups of six Lewis rats were injected with MBPp87–99/CFA to induce active EAE. Just after the onset of each disease (12 days) these rats were administered (intravenously) 2 × 106 W-11 or W-2 CD4+ T cell clones. To ensure the purity of clones W-11 and W-2 that were obtained by limited dilution each clone was subjected to RT-PCR with consensus primers (44), followed by sequencing of the VDJ region. An observer blind to the experimental protocol monitored clinical manifestation of disease. Mean EAE score ± SE of six rats per group is shown.