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The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo
E. Helen Kemp, … , Anthony P. Weetman, Philip F. Watson
E. Helen Kemp, … , Anthony P. Weetman, Philip F. Watson
Published April 1, 2002
Citation Information: J Clin Invest. 2002;109(7):923-930. https://doi.org/10.1172/JCI14643.
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Article Immunology

The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo

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Abstract

Vitiligo is a common depigmenting disorder resulting from the loss of melanocytes in the skin. The pathogenesis of the disease remains obscure, although autoimmune mechanisms are thought to be involved. Indeed, autoantibodies and autoreactive T lymphocytes that target melanocytes have been reported in some vitiligo patients. The objective of this study was to identify pigment cell antigens that are recognized by autoantibodies in vitiligo. Using IgG from vitiligo patients to screen a melanocyte cDNA phage-display library, we identified the melanin-concentrating hormone receptor 1 (MCHR1) as a novel autoantigen related to this disorder. Immunoreactivity against the receptor was demonstrated in vitiligo patient sera by using radiobinding assays. Among sera from healthy controls and from patients with autoimmune disease, none exhibited immunoreactivity to MCHR1, indicating a high disease specificity for Ab’s against the receptor. Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown.

Authors

E. Helen Kemp, Elizabeth A. Waterman, Brian E. Hawes, Kim O’Neill, Raju V.S.R.K. Gottumukkala, David J. Gawkrodger, Anthony P. Weetman, Philip F. Watson

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Figure 4

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Absorption experiments. MCHR1 Ab-positive sera (n = 3) and a pool of hea...
Absorption experiments. MCHR1 Ab-positive sera (n = 3) and a pool of healthy control sera (n = 6) were preabsorbed with either CHO or CHO-MCHR1 cell membranes before analysis in the radiobinding assay. The results of two experiments are shown as the mean Ab index and SD. A pool of control sera (sample 1) and MCHR1 Ab-positive vitiligo patient sera (samples 2–4), each without preincubation (black bars), with preincubation with CHO cell membranes (white bars) and with preincubation with CHO-MCHR1 cell membranes (gray bars).

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ISSN: 0021-9738 (print), 1558-8238 (online)

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