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The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo
E. Helen Kemp, … , Anthony P. Weetman, Philip F. Watson
E. Helen Kemp, … , Anthony P. Weetman, Philip F. Watson
Published April 1, 2002
Citation Information: J Clin Invest. 2002;109(7):923-930. https://doi.org/10.1172/JCI14643.
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Article Immunology

The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo

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Abstract

Vitiligo is a common depigmenting disorder resulting from the loss of melanocytes in the skin. The pathogenesis of the disease remains obscure, although autoimmune mechanisms are thought to be involved. Indeed, autoantibodies and autoreactive T lymphocytes that target melanocytes have been reported in some vitiligo patients. The objective of this study was to identify pigment cell antigens that are recognized by autoantibodies in vitiligo. Using IgG from vitiligo patients to screen a melanocyte cDNA phage-display library, we identified the melanin-concentrating hormone receptor 1 (MCHR1) as a novel autoantigen related to this disorder. Immunoreactivity against the receptor was demonstrated in vitiligo patient sera by using radiobinding assays. Among sera from healthy controls and from patients with autoimmune disease, none exhibited immunoreactivity to MCHR1, indicating a high disease specificity for Ab’s against the receptor. Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown.

Authors

E. Helen Kemp, Elizabeth A. Waterman, Brian E. Hawes, Kim O’Neill, Raju V.S.R.K. Gottumukkala, David J. Gawkrodger, Anthony P. Weetman, Philip F. Watson

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Figure 2

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Radiobinding assays. Vitiligo sera (n = 55), GD patient sera (n = 20) AD...
Radiobinding assays. Vitiligo sera (n = 55), GD patient sera (n = 20) AD patient sera (n = 16), healthy control sera (n = 28) and SLE patient sera (n = 15) were analyzed in radiobinding assays. The Ab index shown for each serum is the mean of at least two experiments. The gray line shows the upper level of normal for the radiobinding assay at an Ab index of 1.43.

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ISSN: 0021-9738 (print), 1558-8238 (online)

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