Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis
Christine Fritsch, … , Marc S. Levin, Deborah C. Rubin
Christine Fritsch, … , Marc S. Levin, Deborah C. Rubin
Published December 1, 2002
Citation Information: J Clin Invest. 2002;110(11):1629-1641. https://doi.org/10.1172/JCI13588.
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Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis

Abstract

Research Article

Authors

Christine Fritsch, Elzbieta A. Swietlicki, Olivier Lefebvre, Michele Kedinger, Hristo Iordanov, Marc S. Levin, Deborah C. Rubin

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Figure 12

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Expression of patched receptor is increased in epimorphin-transfected my...
Expression of patched receptor is increased in epimorphin-transfected myofibroblasts. Caco2, pCEN vector control, or EpiS-transfected cells were harvested for total protein. (a, c) Protein extracts (50 μg per lane) were separated by reduced SDS-PAGE on 4–12% acrylamide gels, transferred to nitrocellulose, incubated with (a)the polyclonal patched antibody or (c) the polyclonal Hsp40 antibody, and then visualized with enhanced chemiluminescence reagent. (b) Bands specific for patched were quantified as indicated in Methods.