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Transplantable hematopoietic stem cells in human fetal liver have a CD34+ side population (SP)phenotype
Naoyuki Uchida, … , Allen C. Eaves, Connie J. Eaves
Naoyuki Uchida, … , Allen C. Eaves, Connie J. Eaves
Published October 1, 2001
Citation Information: J Clin Invest. 2001;108(7):1071-1077. https://doi.org/10.1172/JCI13297.
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Article

Transplantable hematopoietic stem cells in human fetal liver have a CD34+ side population (SP)phenotype

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Abstract

Cells with a verapamil-sensitive ability to efflux Hoechst 33342 (termed side population [SP] cells) have been identified in adult marrow from several species including humans and in several tissues from adult mice. In mice, the SP phenotype appears to be a common feature of stem cells, but human SP cells have been less well characterized. We show here, for the first time to our knowledge, that SP cells are present in the second-trimester human fetal liver. They include all of the transplantable human hematopoietic stem cell activity detectable in NOD/SCID mice and also certain other, more differentiated hematopoietic cell types. Notably, the stem cell activity was confined to the CD34+CD38– SP+ population, and isolation of these cells gave an approximately tenfold enrichment of transplantable stem cells. This subset was not, however, coenriched in hematopoietic progenitors detectable by either short- or long-term in vitro assays, indicating most of these to be distinct from transplantable stem cells. These findings suggest that the SP phenotype is an important and distinguishing property of human hematopoietic stem cells and that early in ontogeny they express CD34.

Authors

Naoyuki Uchida, Tomoaki Fujisaki, Allen C. Eaves, Connie J. Eaves

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Figure 1

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Representative FACS dot plot showing the presence and phenotypes of SP c...
Representative FACS dot plot showing the presence and phenotypes of SP cells in the lin– fraction of human fetal liver cells. Cells were depleted of lin+ cells and stained with Hoechst 33342 and antibodies to CD34 and CD38 as described in Methods. (a) Small gated cell population identifies the SP cells (2.5% ± 1.2% of the total lin– fetal liver population; n = 9) that disappear in the presence of verapamil (b). The distribution of cells according to their expression of CD34 and CD38 in the SP (c) and total (d) fractions of the lin– fetal liver population analyzed here is also shown.

Copyright © 2023 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

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