Immunophenotype of isolated thymic DC subsets analyzed by flow cytometry. Thymic
DCs were sorted into Lin- (PE-Cy5) HLA-DRint (FITC) and Lin-
HLA-DRhi subsets. Anti-CD13-PE-Cy5 labeling of
HLA-DRint cells clearly resolved two distinct populations.
CD13+ HLA-DRint, CD13- HLA-DRint, and
CD13+ HLA-DRhi DCs were analyzed using
PE-conjugated mAb’s for the expression of a number of lymphoid,
myeloid, costimulatory, and adhesion markers. Data shown are representative of
three experiments. Ag, antigen.