JCI - Human thymus contains IFN-α-producing CD11c–, myeloid CD11c+, and mature interdigitating dendritic cells

Human thymus contains IFN-α-producing CD11c^–, myeloid CD11c⁺, and mature interdigitating dendritic cells

Nathalie Bendriss-Vermare, Clarisse Barthélémy, Isabelle Durand, Corine Bruand, Colette Dezutter-Dambuyant, Nathalie Moulian, Sonia Berrih-Aknin, Christophe Caux, Giorgio Trinchieri, Francine Brière

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Immunophenotype of isolated thymic DC subsets analyzed by flow cytometry. Thymic DCs were sorted into Lin- (PE-Cy5) HLA-DR^int (FITC) and Lin- HLA-DR^hi subsets. Anti-CD13-PE-Cy5 labeling of HLA-DR^int cells clearly resolved two distinct populations. CD13⁺ HLA-DRint, CD13- HLA-DR^int, and CD13⁺ HLA-DR^hi DCs were analyzed using PE-conjugated mAb’s for the expression of a number of lymphoid, myeloid, costimulatory, and adhesion markers. Data shown are representative of three experiments. Ag, antigen.

Human thymus contains IFN-α-producing CD11c–, myeloid CD11c+, and mature interdigitating dendritic cells

Human thymus contains IFN-α-producing CD11c–, myeloid CD11c+, and mature interdigitating dendritic cells

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Human thymus contains IFN-α-producing CD11c^–, myeloid CD11c⁺, and mature interdigitating dendritic cells

Human thymus contains IFN-α-producing CD11c^–, myeloid CD11c⁺, and mature interdigitating dendritic cells