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Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue
Anying Song, … , Philipp E. Scherer, Qiong A. Wang
Anying Song, … , Philipp E. Scherer, Qiong A. Wang
Published October 1, 2019
Citation Information: J Clin Invest. 2020;130(1):247-257. https://doi.org/10.1172/JCI129167.
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Research Article Metabolism

Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue

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Abstract

Brown adipose tissue (BAT), as the main site of adaptive thermogenesis, exerts beneficial metabolic effects on obesity and insulin resistance. BAT has been previously assumed to contain a homogeneous population of brown adipocytes. Utilizing multiple mouse models capable of genetically labeling different cellular populations, as well as single-cell RNA sequencing and 3D tissue profiling, we discovered a brown adipocyte subpopulation with low thermogenic activity coexisting with the classical high-thermogenic brown adipocytes within the BAT. Compared with the high-thermogenic brown adipocytes, these low-thermogenic brown adipocytes had substantially lower Ucp1 and Adipoq expression, larger lipid droplets, and lower mitochondrial content. Functional analyses showed that, unlike the high-thermogenic brown adipocytes, the low-thermogenic brown adipocytes have markedly lower basal mitochondrial respiration, and they are specialized in fatty acid uptake. Upon changes in environmental temperature, the 2 brown adipocyte subpopulations underwent dynamic interconversions. Cold exposure converted low-thermogenic brown adipocytes into high-thermogenic cells. A thermoneutral environment had the opposite effect. The recruitment of high-thermogenic brown adipocytes by cold stimulation is not affected by high-fat diet feeding, but it does substantially decline with age. Our results revealed a high degree of functional heterogeneity of brown adipocytes.

Authors

Anying Song, Wenting Dai, Min Jee Jang, Leonard Medrano, Zhuo Li, Hu Zhao, Mengle Shao, Jiayi Tan, Aimin Li, Tinglu Ning, Marcia M. Miller, Brian Armstrong, Janice M. Huss, Yi Zhu, Yong Liu, Viviana Gradinaru, Xiwei Wu, Lei Jiang, Philipp E. Scherer, Qiong A. Wang

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Figure 1

Two subpopulations of classical brown adipocytes undergo dynamic interconversions in vivo.

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Two subpopulations of classical brown adipocytes undergo dynamic interco...
(A) Representative X-gal staining of BAT from AdipoChaser-LacZ mice exposed to different environmental temperatures while fed with dox-containing chow diet. (B) Quantification of the percentage of LacZ+ brown adipocytes in the total brown adipocytes. n = 8 mice (6°C); 6 mice (24°C); 7 mice (30°C). (C–F) Representative X-gal staining of BAT from AdipoChaser-LacZ mice kept at the indicated temperatures while fed with dox-containing chow diet, followed by regular chow diet feeding at the indicated temperatures. Scale bars: 100 μm (A, C–F). All data are mean ± SD of biologically independent samples; **P < 0.01. Statistical significance was assessed using a 1-way ANOVA followed by Tukey’s multiple comparisons test. All images are representative of 3 independent experiments.

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ISSN: 0021-9738 (print), 1558-8238 (online)

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