(A) HLA class I restriction of p53^Y220C (patient 5, TIL culture 4259-F1) and p53^R248W (patient 9, TIL culture 4266-F1) neoepitopes. Data are mean ± SEM, n = 3 technical replicates. > indicates 1,250 pg/ml IFN-γ. (B) Peptide mapping of CD4⁺ T cell responses from patient 4 to p53^R175H neoepitopes. (C) HLA class II restriction for p53^Y220C neoantigen from patient 5. (D) HLA allele frequencies from selected populations (http://www.allelefrequencies.net/default.asp). (E) Specificity of HLA-DRB*13:01/p53^R175H–reactive TCR to 25 amino acid p53^R175H peptides.