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Research Article Free access | 10.1172/JCI119121

Bacterial infection induces nitric oxide synthase in human neutrophils.

M A Wheeler, S D Smith, G García-Cardeña, C F Nathan, R M Weiss, and W C Sessa

Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Find articles by Wheeler, M. in: JCI | PubMed | Google Scholar

Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Find articles by Smith, S. in: JCI | PubMed | Google Scholar

Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Find articles by García-Cardeña, G. in: JCI | PubMed | Google Scholar

Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Find articles by Nathan, C. in: JCI | PubMed | Google Scholar

Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

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Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Find articles by Sessa, W. in: JCI | PubMed | Google Scholar

Published January 1, 1997 - More info

Published in Volume 99, Issue 1 on January 1, 1997
J Clin Invest. 1997;99(1):110–116. https://doi.org/10.1172/JCI119121.
© 1997 The American Society for Clinical Investigation
Published January 1, 1997 - Version history
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Abstract

The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with that in neutrophil-enriched fractions from noninfected controls. Partially purified inducible nitric oxide synthase is primarily membrane associated, calcium independent, and inhibited by arginine analogues with a rank order consistent with that of purified human inducible nitric oxide synthase. Molecular, biochemical, and immunocytochemical evidence unequivocally identifies inducible nitric oxide synthase as the major nitric oxide synthase isoform found in neutrophils isolated from urine during urinary tract infections. Elevated inducible nitric oxide synthase activity and elevated nitric oxide synthase protein measured in patients with urinary tract infections and treated with antibiotics does not decrease until 6-10 d of antibiotic treatment. The extended elevation of neutrophil inducible nitric oxide synthase during urinary tract infections may have both antimicrobial and proinflammatory functions.

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